PE-iFluor® 710 Tandem

Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 710 specific B12-A channel.

The fluorescence intensity of PE-iFluor® 710 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	747

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Absorbance (nm)

566

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

747

PE-iFluor® 710 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~740 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 710 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. It is optimized to provide a new unique color for spectral flow cytometry. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

Spectrum

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Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	747

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
PE-iFluor® 700 Tandem	565	708	1960000
PE-iFluor® 610 Tandem	565	625	1960000
PE-iFluor® 647 Tandem	565	666	1960000
PE-iFluor® 750 Tandem	565	778	1960000
PE-iFluor® 594 Tandem	565	606	1960000
PE-iFluor® 660 Tandem	565	695	1960000
PE-iFluor™ 597 Tandem	565	612	-
PE-iFluor™ 740 Tandem	565	767	1960000
PE-iFluor™ 720 Tandem	565	750	1960000
PE-iFluor™ 770 Tandem	565	795	1960000
APC-iFluor® 710 Tandem	651	747	700000
Show More (2)

Images

Figure 1. Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora flow cytometer in the PE-iFluor® 710 specific B12-A channel.

Figure 2. The fluorescence intensity of PE-iFluor® 710 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.

References

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CUBIC: a three-dimensional colored projection of Consort 30 generated trivariate flow cytometric data.
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R-phycoerythrin-cyanine 5 tandem discerns CD72 polymorphism.
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Journal: Immunogenetics : 307-14