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PE-iFluor® 710 Tandem

PE-iFluor® 710 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~740 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 710 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. It is optimized to provide a new unique color for spectral flow cytometry. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
APC-iFluor® 710 Tandem651747700000
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References

View all 10 references: Citation Explorer
A job for quantum dots: use of a smartphone and 3D-printed accessory for all-in-one excitation and imaging of photoluminescence.
Authors: Petryayeva, Eleonora and Algar, W Russ
Journal: Analytical and bioanalytical chemistry (2016): 2913-25
Light tolerance of R-phycoerythrin and a tandem conjugate observed by single molecule recrossing events.
Authors: Burrows, Sean M and Patel, Payal and Pappas, Dimitri
Journal: Applied spectroscopy (2009): 709-15
Flow cytometric immunophenotyping including Bcl-2 detection on fine needle aspirates in the diagnosis of reactive lymphadenopathy and non-Hodgkin's lymphoma.
Authors: Laane, Edward and Tani, Edneia and Björklund, Elisabet and Elmberger, Göran and Everaus, Hele and Skoog, Lambert and Porwit-MacDonald, Anna
Journal: Cytometry. Part B, Clinical cytometry (2005): 34-42
Validation of a flow cytometric assay detecting in vitro basophil activation for the diagnosis of muscle relaxant allergy.
Authors: Abuaf, N and Rajoely, B and Ghazouani, E and Levy, D A and Pecquet, C and Chabane, H and Leynadier, F
Journal: The Journal of allergy and clinical immunology (1999): 411-8
A strategy for multiple immunophenotyping by image cytometry: model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes.
Authors: Gothot, A and Grosdent, J C and Paulus, J M
Journal: Cytometry (1996): 214-25
Page updated on December 13, 2024

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Catalog Number2615
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Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)

566

Extinction coefficient (cm -1 M -1)

1960000

Excitation (nm)

565

Emission (nm)

747

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 710 specific B12-A channel.
Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 710 specific B12-A channel.
Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 710 specific B12-A channel.
The fluorescence intensity of PE-iFluor® 710 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.