PE-iFluor® 710 Tandem

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	747

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Absorbance (nm)

566

Extinction coefficient (cm ^-1 M ^-1)

1960000

Excitation (nm)

565

Emission (nm)

747

PE-iFluor® 710 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~740 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 710 Tandem gives a significantly improved staining index than the corresponding PE-Alexa Fluor™ 700 Tandem. It is optimized to provide a new unique color for spectral flow cytometry. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

Spectrum

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Spectral properties

Absorbance (nm)	566
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	747

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
PE-iFluor® 700 Tandem	565	708	1960000
PE-iFluor® 610 Tandem	565	625	1960000
PE-iFluor® 647 Tandem	565	666	1960000
PE-iFluor® 750 Tandem	565	778	1960000
PE-iFluor® 594 Tandem	565	606	1960000
PE-iFluor® 660 Tandem	565	695	1960000
PE-iFluor® 780 Tandem	566	575	1960000
PE-iFluor® 597 Tandem	565	612	-
PE-iFluor® 740 Tandem	565	767	1960000
PE-iFluor® 720 Tandem	565	750	1960000
PE-iFluor® 770 Tandem	567	575	1960000
APC-iFluor® 710 Tandem	651	747	700000
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Images

Figure 1. Flow cytometry analysis of PBMC stained with PE-iFluor® 710 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 710 specific B12-A channel.

Figure 2. The fluorescence intensity of PE-iFluor® 710 anti-human CD4 *SK3* conjugate at different concentrations in the range of 0.03125 to 0.5 µg. Results showed that the fluorescence intensity of the CD conjugates remained nearly consistent.

References

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Journal: Analytical and bioanalytical chemistry (2016): 2913-25

Light tolerance of R-phycoerythrin and a tandem conjugate observed by single molecule recrossing events.
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Journal: Applied spectroscopy (2009): 709-15

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Journal: Cytometry (1996): 214-25

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Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1995): 103-6

Application of a flow cytometric method using autofluorescence and a tandem fluorescent dye to analyze human alveolar macrophage surface markers.
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Journal: Journal of immunological methods (1994): 17-24

CUBIC: a three-dimensional colored projection of Consort 30 generated trivariate flow cytometric data.
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Journal: Cytometry (1991): 570-8

Single laser flow cytometric detection of lymphocytes binding three antibodies labelled with fluorescein, phycoerythrin and a novel tandem fluorochrome conjugate.
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Journal: Journal of immunological methods (1990): 69-78

R-phycoerythrin-cyanine 5 tandem discerns CD72 polymorphism.
Authors: Doucet, M and Soussi, N and Crain-Denoyelle, A M and Gendron, M C and Sanchez, P
Journal: Immunogenetics : 307-14