Name: PE-iFluor® 750 Tandem
Brand: AAT Bioquest
SKU: 2704
Price: 237 USD
Availability: InStock

PE-iFluor® 750 Tandem

PE-iFluor® 750 Tandem is a PE-based tandem fluorophore that uses phycoerythrin (PE) as the donor and iFluor® 750 as the acceptor dye for near-infrared fluorescence detection in flow cytometry.

Detection Principle: Uses fluorescence resonance energy transfer (FRET) between PE and iFluor® 750.
Spectral Properties: Excitation/emission maxima of 565/778 nm.
Enhanced Staining Performance: Provides a higher staining index than PE-Alexa Fluor® 750 and PE-Cy7 tandems.
Instrument Compatibility: Validated on the Cytek Aurora spectral flow cytometer.
Applications: Suitable for multicolor and spectral flow cytometry.

Flow cytometry analysis of whole blood stained with PE-iFluor® 750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 750 specific B13-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2704	1 mg	Price

Overview

PE-iFluor® 750 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~770 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 750 Tandem gives an improved staining index than the corresponding PE-Alexa Fluor™ 750 or PE-Cy7 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

References

View all 33 references: Citation Explorer

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.

Authors:

Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno

Journal:

Cytometry. Part B, Clinical cytometry (2021): 194-205

Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.

Authors:

Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)

Probing Surface Membrane Receptors Using Engineered Bacteriophage Bioconjugates.

Authors:

Lomakin, Yakov A and Kaminskaya, Alena N and Stepanov, Alexey V and Shmidt, Anna A and Gabibov, Alexander G and Belogurov, Alexey A

Journal:

Bioconjugate chemistry (2019): 1500-1506

Initial flow cytometric evaluation of the Clearllab lymphoid screen.

Authors:

Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L

Journal:

Cytometry. Part B, Clinical cytometry (2018): 707-713

Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid.

Authors:

Baronio, Diego and Bauer-Negrini, Guilherme and Castro, Kamila and Della-Flora Nunes, Gustavo and Riesgo, Rudimar and Mendes-da-Cruz, Daniella Arêas and Savino, Wilson and Gottfried, Carmem and Bambini-Junior, Victorio

Journal:

Neuroimmunomodulation (2018): 280-284

Physical properties

Molecular weight	~240000
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	778

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on November 12, 2026