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PE-iFluor® 750 Tandem

Flow cytometry analysis of whole blood stained with PE-iFluor® 750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 750 specific B13-A channel.
Flow cytometry analysis of whole blood stained with PE-iFluor® 750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 750 specific B13-A channel.
Flow cytometry analysis of whole blood stained with PE-iFluor® 750 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 750 specific B13-A channel.
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Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)778
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)
565
Emission (nm)
778
PE-iFluor® 750 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~770 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 750 Tandem gives an improved staining index than the corresponding PE-Alexa Fluor™ 750 or PE-Cy7 Tandem. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

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Spectral properties

Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)565
Emission (nm)778

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 780 Tandem5665751960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
APC-iFluor® 750 Tandem651776700000
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References


View all 33 references: Citation Explorer
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Probing Surface Membrane Receptors Using Engineered Bacteriophage Bioconjugates.
Authors: Lomakin, Yakov A and Kaminskaya, Alena N and Stepanov, Alexey V and Shmidt, Anna A and Gabibov, Alexander G and Belogurov, Alexey A
Journal: Bioconjugate chemistry (2019): 1500-1506
Initial flow cytometric evaluation of the Clearllab lymphoid screen.
Authors: Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L
Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713
Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid.
Authors: Baronio, Diego and Bauer-Negrini, Guilherme and Castro, Kamila and Della-Flora Nunes, Gustavo and Riesgo, Rudimar and Mendes-da-Cruz, Daniella Arêas and Savino, Wilson and Gottfried, Carmem and Bambini-Junior, Victorio
Journal: Neuroimmunomodulation (2018): 280-284
A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143
CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Comparison of Flow-cytometric Antibody Secreting Cell Assay and Mabtech Immunoglobulin ELISpot Assay.
Authors: Lee, N and In, J W and Kim, H and Roh, E Y and Shin, S and Park, K U and Yang, J and Song, E Y
Journal: Transplantation proceedings (2017): 963-966
[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965