PE-iFluor® 770 Tandem

PE-iFluor® 770 Tandem is a PE-based tandem fluorophore that uses phycoerythrin (PE) as the donor and iFluor® 770 as the acceptor dye for near-infrared fluorescence detection in flow cytometry.

Detection Principle: Uses fluorescence resonance energy transfer (FRET) between PE and iFluor® 770.
Spectral Properties: Excitation/emission maxima of 567/803 nm.
Enhanced Staining Performance: Provides a significantly higher staining index than spectrally similar PE-IR dye tandems.
Instrument Compatibility: Validated on the Cytek Aurora spectral flow cytometer.
Applications: Suitable for multicolor and spectral flow cytometry.

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature.
(Bottom) Flow cytometry analysis of whole blood stained with PE-iFluor® 770 Anti-human CD4 (Clone: SK3) conjugate. The fluorescence signal was monitored using a Cytek Aurora spectral flow cytometer in the PE-iFluor® 770-specific B14-A channel.

Datasheet

SDS

Catalog	Size	Price	Quantity
2608	1 mg	Price

Overview

PE-iFluor® 770 Tandem is a new color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~800 nm. It has been validated with a spectral flow cytometer. PE-iFluor® 770 Tandem gives a significantly improved staining index than the other sepctrally similar PE-IR dye tandems. It is optimized to provide a new unique color for spectral flow cytometry. AAT Bioquest offer the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

References

View all 18 references: Citation Explorer

The Dual-Targeted Peptide Conjugated Probe for Depicting Residual Nasopharyngeal Carcinoma and Guiding Surgery.

Authors:

Huang, Wenhui and He, Zicong and Cai, Xuekang and Zhang, Jingming and Li, Wei and Wang, Kun and Zhang, Shuixing

Journal:

Biosensors (2022)

Deep learning for in vivo near-infrared imaging.

Authors:

Ma, Zhuoran and Wang, Feifei and Wang, Weizhi and Zhong, Yeteng and Dai, Hongjie

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2021)

Measuring Kidney Perfusion, pH, and Renal Clearance Consecutively Using MRI and Multispectral Optoacoustic Tomography.

Authors:

Minhas, Atul S and Sharkey, Jack and Randtke, Edward A and Murray, Patricia and Wilm, Bettina and Pagel, Mark D and Poptani, Harish

Journal:

Molecular imaging and biology (2020): 494-503

Fast DNA Extraction with Polyacrylamide Microspheres for Polymerase Chain Reaction Detection.

Authors:

Wang, Jun and Zhang, Shuyan and Xu, Xinhui and Xing, Yujun and Li, Zongru and Wang, Jinke

Journal:

ACS omega (2020): 13829-13839

Near-infrared fluorescence laparoscopy of the ureter with three preclinical dyes in a pig model.

Authors:

van den Bos, Jacqueline and Al-Taher, Mahdi and Bouvy, Nicole D and Stassen, Laurents P S

Journal:

Surgical endoscopy (2019): 986-991

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	567
Emission (nm)	803

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on November 7, 2026