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PE-iFluor® 780 Tandem

Flow cytometry analysis of PBMC stained with PE-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 780 specific B14-A channel.
Flow cytometry analysis of PBMC stained with PE-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 780 specific B14-A channel.
Flow cytometry analysis of PBMC stained with PE-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE-iFluor® 780 specific B14-A channel.
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Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)566
Emission (nm)575
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)
566
Emission (nm)
575
PE-iFluor® 780 Tandem is a new NIR color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~800 nm. It has been validated with a spectral flow cytometer. It is optimized to provide a new unique color for spectral flow cytometry. AAT Bioquest offers the largest number of colors for conventional and spectral flow cytometry applications, including iFluor®, mFluor™ small organic dyes and a variety of their tandems.

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Spectral properties

Extinction coefficient (cm -1 M -1)1960000
Excitation (nm)566
Emission (nm)575

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-iFluor® 700 Tandem5657081960000
PE-iFluor® 610 Tandem5656251960000
PE-iFluor® 647 Tandem5656661960000
PE-iFluor® 750 Tandem5657781960000
PE-iFluor® 710 Tandem5657471960000
PE-iFluor® 594 Tandem5656061960000
PE-iFluor® 660 Tandem5656951960000
PE-iFluor® 597 Tandem565612-
PE-iFluor® 740 Tandem5657671960000
PE-iFluor® 720 Tandem5657501960000
PE-iFluor® 770 Tandem5675751960000
APC-iFluor® 780 Tandem787812700000
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References


View all 4 references: Citation Explorer
Tandem dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2014): 164-74
Tandem Dyes: Stability in cocktails and compensation considerations.
Authors: Johansson, Ulrika and Macey, Marion
Journal: Cytometry. Part B, Clinical cytometry (2013)
Flow cytometry and the stability of phycoerythrin-tandem dye conjugates.
Authors: Hulspas, Ruud and Dombkowski, David and Preffer, Frederic and Douglas, Derick and Kildew-Shah, Brian and Gilbert, John
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 966-72
Near-infrared dyes for six-color immunophenotyping by laser scanning cytometry.
Authors: Gerstner, Andreas O H and Lenz, Dominik and Laffers, Wiebke and Hoffman, Robert A and Steinbrecher, Michael and Bootz, Friedrich and Tárnok, Attila
Journal: Cytometry (2002): 115-23