Phycoerythrin (PE)
R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The relative prominence of the secondary peaks varies significantly among R-PEs from different species. PE has three types of subunits: alpha (20,000 daltons), beta (20,000 daltons) and gamma (30,000 daltons). The molecular weight of intact PE has been found to be about 240,000 daltons. The alpha subunit of PE contains only the phycoerythrobilin (PEB) chromophore, while beta and gamma subunits contain both PEB and phycourobilin (PUB). Variability in the absorption spectra of PEs from various species reflects differences in the PEB/PUB ratio of the subunits. PE and closely related B-PE are the most intensely fluorescent phycobiliproteins, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free* | 565 | 574 | 1960000 | 0.82 | 0.175 |
Citations
View all 5 citations: Citation Explorer
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Authors: Fan, Lin and Liu, Junwei and Hu, Wei and Chen, Zexin and Lan, Jie and Zhang, Tongtong and Zhang, Yang and Wu, Xianpeng and Zhong, Zhiwei and Zhang, Danyang and others,
Journal: Cell Research (2024): 1--21
Authors: Fan, Lin and Liu, Junwei and Hu, Wei and Chen, Zexin and Lan, Jie and Zhang, Tongtong and Zhang, Yang and Wu, Xianpeng and Zhong, Zhiwei and Zhang, Danyang and others,
Journal: Cell Research (2024): 1--21
Direct Cytosolic Delivery of Proteins and CRISPR-Cas9 Genome Editing by Gemini Amphiphiles via Non-Endocytic Translocation Pathways
Authors: Le, Zhicheng and Pan, Qi and He, Zepeng and Liu, Hong and Shi, Yi and Liu, Lixin and Liu, Zhijia and Ping, Yuan and Chen, Yongming
Journal: ACS Central Science (2023)
Authors: Le, Zhicheng and Pan, Qi and He, Zepeng and Liu, Hong and Shi, Yi and Liu, Lixin and Liu, Zhijia and Ping, Yuan and Chen, Yongming
Journal: ACS Central Science (2023)
CD169+ subcapsular sinus macrophage-derived microvesicles are associated with light zone follicular dendritic cells
Authors: Chen, Xin and Zheng, Yuhan and Liu, Siming and Yu, Wenjing and Liu, Zhiduo
Journal: European Journal of Immunology (2022): 1581--1594
Authors: Chen, Xin and Zheng, Yuhan and Liu, Siming and Yu, Wenjing and Liu, Zhiduo
Journal: European Journal of Immunology (2022): 1581--1594
Nanobubbles Containing sPD-1 and Ce6 Mediate Combination Immunotherapy and Suppress Hepatocellular Carcinoma in Mice
Authors: Tan, Yandi and Yang, Shiqi and Ma, Yao and Li, Jinlin and Xie, Qian and Liu, Chaoqi and Zhao, Yun
Journal: International Journal of Nanomedicine (2021): 3241
Authors: Tan, Yandi and Yang, Shiqi and Ma, Yao and Li, Jinlin and Xie, Qian and Liu, Chaoqi and Zhao, Yun
Journal: International Journal of Nanomedicine (2021): 3241
Morphological Changes Induced By Extremely Low-Frequency Electric Fields
Authors: Imani, Mahdi and Kazemi, Sepide and Saviz, Mehrdad and Farahm, undefined and , Leila and Sadeghi, Behnam and Faraji-dana, Reza
Journal: Bioelectromagnetics (2019)
Authors: Imani, Mahdi and Kazemi, Sepide and Saviz, Mehrdad and Farahm, undefined and , Leila and Sadeghi, Behnam and Faraji-dana, Reza
Journal: Bioelectromagnetics (2019)
References
View all 46 references: Citation Explorer
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Journal: J Biol Chem (2006): 8573
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Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
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