PE [R-Phycoerythrin] *CAS 11016-17-4*

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<p>Phycoerythrin (PE) is a red protein-pigment complex from the light harvesting phycobiliprotein family, present in red algae and cryptophytes, accessory to the main chlorophyll pigments responsible for photosynthesis. Like all phycobiliproteins, it is composed of a protein part covalently binding chromophores called phycobilins. In the phycoerythrin family, the most known phycobilins are: phycoerythrobilin, the typical phycoerythrin acceptor chromophore, and sometimes phycourobilin. Phycoerythrins are composed of (αβ) monomers, usually organized in a disk-shaped trimer (αβ)<sub>3</sub> or hexamer (αβ)<sub>6</sub> (second one is the functional unit of the antenna rods). These typical complexes also contain a third type of subunit, the γ chain. Phycobilin pigments have excellent fluorescent properties that are extremely useful for flow cytometry-based immunoassays.</p>
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Overview

Ex/Em (nm)564/574
CAS #11016-17-4
SolventWater
Storage Refrigerated (2-8 °C)
Minimize light exposure
Category Protein-Based Tags
PE and APC
Related Protein Dyes
R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. The relative prominence of the secondary peaks varies significantly among R-PEs from different species. PE has three types of subunits: alpha (20,000 daltons), beta (20,000 daltons) and gamma (30,000 daltons). The molecular weight of intact PE has been found to be about 240,000 daltons. The alpha subunit of PE contains only the phycoerythrobilin (PEB) chromophore, while beta and gamma subunits contain both PEB and phycourobilin (PUB). Variability in the absorption spectra of PEs from various species reflects differences in the PEB/PUB ratio of the subunits. PE and closely related B-PE are the most intensely fluorescent phycobiliproteins, with quantum efficiencies probably in excess of 90%, and its orange fluorescence is readily visible by eye in any moderately concentrated solution.




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References

Morphological Changes Induced By Extremely Low-Frequency Electric Fields
Authors: Mahdi Imani, Sepide Kazemi, Mehrdad Saviz, Leila Farahmand, Behnam Sadeghi, Reza Faraji-dana
Journal: Bioelectromagnetics (2019)






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