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PerCP-Cy5.5 Tandem

Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.
Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.
Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.
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Physical properties
Molecular weightN/A
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)
482
Emission (nm)
695
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)482
Emission (nm)695

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-Cy5.5 Tandem5656711960000
APC-Cy5.5 Tandem651700700000

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References


View all 2 references: Citation Explorer
Four color compensation.
Authors: Stewart, C C and Stewart, S J
Journal: Cytometry (1999): 161-75
A strategy for multiple immunophenotyping by image cytometry: model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes.
Authors: Gothot, A and Grosdent, J C and Paulus, J M
Journal: Cytometry (1996): 214-25