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PerCP-Cy5.5 Tandem

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its tandem conjugates (such as PerCP-Cy5.5) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PE-Cy5.5 Tandem5656711960000
APC-Cy5.5 Tandem651700700000

References

View all 2 references: Citation Explorer
Four color compensation.
Authors: Stewart, C C and Stewart, S J
Journal: Cytometry (1999): 161-75
A strategy for multiple immunophenotyping by image cytometry: model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes.
Authors: Gothot, A and Grosdent, J C and Paulus, J M
Journal: Cytometry (1996): 214-25
Page updated on December 13, 2024

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Catalog Number2650
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Physical properties

Molecular weight

N/A

Solvent

Water

Spectral properties

Extinction coefficient (cm -1 M -1)

350000

Excitation (nm)

482

Emission (nm)

695

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.
Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.
Flow cytometry analysis of whole blood stained with PerCP-Cy5.5 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy5.5 specific B9-A channel.