Calculator
PerCP-Cy7
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 350000 |
| Excitation (nm) | 757 |
| Emission (nm) | 783 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 350000 | Excitation (nm) 757 | Emission (nm) 783 |
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). PerCP-Cy7 tandem conjugate is a common color used in flow cytometry. It can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP-Cy7 conjugates an extremely useful fluorochrome for multicolor analysis with FITC, PE and other fluorochromes.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 350000 |
| Excitation (nm) | 757 |
| Emission (nm) | 783 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
| Cy7 tyramide | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 | 0.0005 | 0.0193 | 0.165 |
| Cy7 Styramide *Superior Replacement for Cy7 tyramide* | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 | 0.0005 | 0.0193 | 0.165 |
| Cy7 tetrazine | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 | 0.0005 | 0.0193 | 0.165 |
Images
Figure 1. Flow cytometry analysis of PBMC stained with PerCP-Cy7 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-Cy7 specific B14-A channel.
References
View all 16 references: Citation Explorer
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Comparison of minimal residual disease detection in multiple myeloma by SRL 8-color single-tube and EuroFlow 8-color 2-tube multiparameter flow cytometry.
Authors: Takamatsu, Hiroyuki and Yoroidaka, Takeshi and Fujisawa, Momoko and Kobori, Kazuya and Hanawa, Masako and Yamashita, Takeshi and Murata, Ryoichi and Ueda, Mikio and Nakao, Shinji and Matsue, Kosei
Journal: International journal of hematology (2019): 377-381
Authors: Takamatsu, Hiroyuki and Yoroidaka, Takeshi and Fujisawa, Momoko and Kobori, Kazuya and Hanawa, Masako and Yamashita, Takeshi and Murata, Ryoichi and Ueda, Mikio and Nakao, Shinji and Matsue, Kosei
Journal: International journal of hematology (2019): 377-381
[Clinical features of imbalance between Th1 and Th22 cells and its association with disease progression in patients with liver cirrhosis].
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
Authors: Wu, H Q and Zhao, J J and Li, H W and Zhang, Z
Journal: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2017): 738-744
[THE STANDARD VALUES OF SUB-POPULATIONS OF T-HELPERS OF DIFFERENT LEVEL OF DIFFERENTIATION IN PERIPHERAL BLOOD].
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Authors: Kudryavtsev, I V and Serebryakova, M K and Totolyan, A A
Journal: Klinicheskaia laboratornaia diagnostika (2016): 179-84
Inhibition of histone deacetylase 6 restores innate immune cells in the bone marrow in a lethal septic model.
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1
Authors: Zhao, Ting and Li, Yongqing and Liu, Baoling and Pan, Baihong and Cheng, Xin and Georgoff, Patrick and Alam, Hasan B
Journal: The journal of trauma and acute care surgery (2016): 34-40; discussion 40-1
Flow cytometric analysis of T lymphocyte proliferation in vivo by EdU incorporation.
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Authors: Sun, Xiaojing and Zhang, Chunpan and Jin, Hua and Sun, Guangyong and Tian, Yue and Shi, Wen and Zhang, Dong
Journal: International immunopharmacology (2016): 56-65
Using six-colour flow cytometry to analyse the activation and interaction of platelets and leukocytes--A new assay suitable for bench and bedside conditions.
Authors: Granja, Tiago and Schad, Jessica and Schüssel, Patricia and Fischer, Claudius and Häberle, Helene and Rosenberger, Peter and Straub, Andreas
Journal: Thrombosis research (2015): 786-96
Authors: Granja, Tiago and Schad, Jessica and Schüssel, Patricia and Fischer, Claudius and Häberle, Helene and Rosenberger, Peter and Straub, Andreas
Journal: Thrombosis research (2015): 786-96
Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53