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PerCP-iFluor® 780

Flow cytometry analysis of PBMC stained with PerCP-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 780 specific B14-A channel.
Flow cytometry analysis of PBMC stained with PerCP-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 780 specific B14-A channel.
Flow cytometry analysis of PBMC stained with PerCP-iFluor® 780 anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PerCP-iFluor® 780 specific B14-A channel.
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Physical properties
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)778
Emission (nm)806
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure

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Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)
778
Emission (nm)
806
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). PerCP-iFluor® 780 tandem conjugates can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP-iFluor® 780 conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. This PerCP tandem might have the longest emission among all the PerCP tandems.

Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)778
Emission (nm)806

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
PerCP-iFluor® 700675707350000
PerCP-iFluor® 710719747350000
iFluor® 780 maleimide7848082500001

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References


View all 3 references: Citation Explorer
A Novel Theranostic Nanoprobe for In Vivo Singlet Oxygen Detection and Real-Time Dose-Effect Relationship Monitoring in Photodynamic Therapy.
Authors: Wang, Han and Wang, Zhaohui and Li, Yongkuan and Xu, Tian and Zhang, Qi and Yang, Man and Wang, Peng and Gu, Yueqing
Journal: Small (Weinheim an der Bergstrasse, Germany) (2019): e1902185
Three-dimensional cellular imaging in thick biological tissue with confocal detection of one-photon fluorescence in the near-infrared II window.
Authors: Wang, Menghan and Chen, Nanguang
Journal: Journal of biophotonics (2019): e201800459
Bright quantum dots emitting at ∼1,600 nm in the NIR-IIb window for deep tissue fluorescence imaging.
Authors: Zhang, Mingxi and Yue, Jingying and Cui, Ran and Ma, Zhuoran and Wan, Hao and Wang, Feifei and Zhu, Shoujun and Zhou, Ying and Kuang, Yun and Zhong, Yeteng and Pang, Dai-Wen and Dai, Hongjie
Journal: Proceedings of the National Academy of Sciences of the United States of America (2018): 6590-6595