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PerCP
Peridinin-Chlorophyll-Protein Complex
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its cyanine tandem conjugates (such as PerCP-Cy5.5 developed by BD) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers iFluor protein labeling dyes that are generally brighter and more photostable than the corresponding cyanine dyes of similar wavelengths. iFluor protein labeling dyes are superior alternatives to the cyanine dyes.
<p>Peridinin chlorophyll (PerCP) is an&nbsp;apocarotenoid&nbsp;pigment&nbsp;that some organisms use in&nbsp;photosynthesis. Many photosynthetic&nbsp;dinoflagellates&nbsp;use peridinin, which absorbs blue-green light in the 470-550 nm range, outside the range accessible to&nbsp;chlorophyll&nbsp;molecules. The&nbsp;peridinin-chlorophyll-protein complex&nbsp;is a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll. PerCP is commonly used in&nbsp;immunoassays&nbsp;such as&nbsp;fluorescence-activated cell sorting&nbsp;(FACS) and&nbsp;flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.</p>
<p>Peridinin chlorophyll (PerCP) is an&nbsp;apocarotenoid&nbsp;pigment&nbsp;that some organisms use in&nbsp;photosynthesis. Many photosynthetic&nbsp;dinoflagellates&nbsp;use peridinin, which absorbs blue-green light in the 470-550 nm range, outside the range accessible to&nbsp;chlorophyll&nbsp;molecules. The&nbsp;peridinin-chlorophyll-protein complex&nbsp;is a specialized molecular complex consisting of a boat-shaped protein molecule with a large central cavity that contains peridinin, chlorophyll, and lipid molecules, usually in a 4:1 ratio of peridinin to chlorophyll. PerCP is commonly used in&nbsp;immunoassays&nbsp;such as&nbsp;fluorescence-activated cell sorting&nbsp;(FACS) and&nbsp;flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.</p>
protocol
Protocol
COA
CatalogSize
Price
Quantity
254010 mg
Price
25591 mg
Price
 
Physical properties
Molecular weight~35000
SolventWater
Spectral properties
Correction factor (280 nm)0.22
Extinction coefficient (cm -1 M -1)
406000
Excitation (nm)477
Emission (nm)678
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 9, 2025