logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
PG-cholesterylamine
PG-cholesterylamine was reported by Zhang et al. to be an excellent fluorescent probe for monitoring cholesterol efflux. Reverse cholesterol transport is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. PG-cholesterylamine is a derivative of N-alkyl-3β-cholesterylamine labeled with a fluorescein derivative, Pennsylvania Green (PG). Compared with the traditional radioisotope-based assay, this fluorescent probe gave similar results in the presence of known modulators of cholesterol efflux, such as cyclic AMP, and different cholesterol acceptors. When the fluorescent probe was employed in a high-throughput screening format, a variety of chemicals and bioactive compounds with known and unknown effects on cholesterol efflux could be tested simultaneously by plate-reader in a short period of time. Treatment of THP-1-derived macrophages with inhibitors of the membrane transporter ATP-binding cassette A1, such as glyburide or a specific antibody, significantly reduced the export of this fluorescent compound, indicating that ATP-binding cassette A1 represents the primary mediator of its cellular efflux. PG-cholesterylamine provides a safe, sensitive and reproducible alternative to radioactive assays in efflux experiments.
<p>RAW 264.7 cells were incubated with 10 uM of PG-Chol for 24 hours in a 96-well plate. The cells were then incubated in 1% FBS medium for over night. The next day, the cholesterol efflux was induced by 200 ug/mL HDL for different period of time. The cell culture medium was collected and fluorescence was measured by a microplate reader at 490/520 nm.</p>
<p>RAW 264.7 cells were incubated with 10 uM of PG-Chol for 24 hours in a 96-well plate. The cells were then incubated in 1% FBS medium for over night. The next day, the cholesterol efflux was induced by 200 ug/mL HDL for different period of time. The cell culture medium was collected and fluorescence was measured by a microplate reader at 490/520 nm.</p>
CatalogSize
Price
Quantity
367001 mg
Price
 
Physical properties

Molecular weight1043.73
SolventDMSO
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS1264272-41-4
Contact us

Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 13, 2025