Name: PG-cholesterylamine
Brand: AAT Bioquest
SKU: 36700
Price: 582 USD
Availability: InStock

PG-cholesterylamine

PG-cholesterylamine was reported by Zhang et al. to be an excellent fluorescent probe for monitoring cholesterol efflux. Reverse cholesterol transport is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. PG-cholesterylamine is a derivative of N-alkyl-3β-cholesterylamine labeled with a fluorescein derivative, Pennsylvania Green (PG). Compared with the traditional radioisotope-based assay, this fluorescent probe gave similar results in the presence of known modulators of cholesterol efflux, such as cyclic AMP, and different cholesterol acceptors. When the fluorescent probe was employed in a high-throughput screening format, a variety of chemicals and bioactive compounds with known and unknown effects on cholesterol efflux could be tested simultaneously by plate-reader in a short period of time. Treatment of THP-1-derived macrophages with inhibitors of the membrane transporter ATP-binding cassette A1, such as glyburide or a specific antibody, significantly reduced the export of this fluorescent compound, indicating that ATP-binding cassette A1 represents the primary mediator of its cellular efflux. PG-cholesterylamine provides a safe, sensitive and reproducible alternative to radioactive assays in efflux experiments.

<p>RAW 264.7 cells were incubated with 10 uM of PG-Chol for 24 hours in a 96-well plate. The cells were then incubated in 1% FBS medium for over night. The next day, the cholesterol efflux was induced by 200 ug/mL HDL for different period of time. The cell culture medium was collected and fluorescence was measured by a microplate reader at 490/520 nm.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
36700	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of PG-cholesterylamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.81 µL	479.051 µL	958.102 µL	4.791 mL	9.581 mL
5 mM	19.162 µL	95.81 µL	191.62 µL	958.102 µL	1.916 mL
10 mM	9.581 µL	47.905 µL	95.81 µL	479.051 µL	958.102 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1043.73 g/mol	=	mL	x	mM	=	mmol

References

View all 6 references: Citation Explorer

High-density lipoprotein cholesterol efflux capacity is inversely associated with cardiovascular risk: a systematic review and meta-analysis

Authors:

Qiu, C.; Zhao, X.; Zhou, Q.; Zhang, Z.

Journal:

Lipids Health Dis (2017): 212

and other factors affecting HDL cholesterol efflux

Authors:

Ronsein, G. E.; Vaisar, T., Inflammation, remodeling

Journal:

Curr Opin Lipidol (2017): 52-59

SR-B1: A Unique Multifunctional Receptor for Cholesterol Influx and Efflux

Authors:

Shen, W. J.; Azhar, S.; Kraemer, F. B.

Journal:

Annu Rev Physiol (2017)

CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells

Authors:

Sun, R. L.; Huang, C. X.; Bao, J. L.; Jiang, J. Y.; Zhang, B.; Zhou, S. X.; Cai, W. B.; Wang, H.; Wang, J. F.; Zhang, Y. L.

Journal:

J Biol Chem (2016): 19532-44

Homocysteine-mediated cholesterol efflux via ABCA1 and ACAT1 DNA methylation in THP-1 monocyte-derived foam cells

Authors:

Liang, Y.; Yang, X.; Ma, L.; Cai, X.; Wang, L.; Yang, C.; Li, G.; Zhang, M.; Sun, W.; Jiang, Y.

Journal:

Acta Biochim Biophys Sin (Shanghai) (2013): 220-8

Physical properties

Molecular weight	1043.73
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	1264272-41-4

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Page updated on October 15, 2025