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AAT Bioquest

Phalloidin lysine

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Physical properties
Molecular weight885.91
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
885.91
Phalloidin lysine is a convenient building block that can be used for developing a variety of phalloidin derivatives that may be for studying cellular structures and other biological applications. It readily reacts with amine-reactive dyes, biotins and other tag molecules (such as NHS esters, isothiocyanates and sulfonyl chlorides etc). Phalloidin, a bicyclic heptapeptide toxin, binds specifically at the interface between F-actin subunits, locking adjacent subunits together. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Phalloidin lysine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM112.878 µL564.391 µL1.129 mL5.644 mL11.288 mL
5 mM22.576 µL112.878 µL225.757 µL1.129 mL2.258 mL
10 mM11.288 µL56.439 µL112.878 µL564.391 µL1.129 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*34344119000-0.250.19
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*499520730000.9210.30.11
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*6506712390000.3310.000.03

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References


View all 7 references: Citation Explorer
Purinergic Signaling and Aminoglycoside Ototoxicity: The Opposing Roles of P1 (Adenosine) and P2 (ATP) Receptors on Cochlear Hair Cell Survival.
Authors: Lin, Shelly C Y and Thorne, Peter R and Housley, Gary D and Vlajkovic, Srdjan M
Journal: Frontiers in cellular neuroscience (2019): 207
Hybrid elastomer-plastic microfluidic device as a convenient model for mimicking the blood-brain barrier in vitro.
Authors: Nguyen, Phuoc Quang Huy and Duong, Duong Duy and Kwun, Jun Dae and Lee, Nae Yoon
Journal: Biomedical microdevices (2019): 90
Gross anatomy of the muscle systems and associated innervation of Apatemon cobitidis proterorhini metacercaria (Trematoda: Strigeidea), as visualized by confocal microscopy.
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Journal: Parasitology (2003): 273-82
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Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1998): 193-203
Expression of PAC 1, an epitope associated with two synapse-enriched glycoproteins and a neuronal cytoskeleton-associated polypeptide in developing forebrain neurons.
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Journal: Neuroscience (1994): 115-29
Vasoactive amines directly modify endothelial cells to affect polymorphonuclear leukocyte diapedesis in vitro.
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Journal: Blood (1987): 1563-9