Name: Phalloidin-PEG4-DBCO
Brand: AAT Bioquest
SKU: 5303
Price: 332 USD
Availability: InStock

Phalloidin-PEG4-DBCO

Phalloidin, a bicyclic heptapeptide toxin, binds specifically at the interface between F-actin subunits, locking adjacent subunits together. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Thus, phalloidin traps actin monomers in a conformation distinct from G-actin and it stabilizes the structure of F-actin by greatly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications. Phalloidin-PEG4-DBCO can be readily used for conjugations with azido-containing molecules.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5303	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Phalloidin-PEG4-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	73.177 µL	365.885 µL	731.77 µL	3.659 mL	7.318 mL
5 mM	14.635 µL	73.177 µL	146.354 µL	731.77 µL	1.464 mL
10 mM	7.318 µL	36.588 µL	73.177 µL	365.885 µL	731.77 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1366.55 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates

Authors:

Hagan, I. M.

Journal:

Cold Spring Harb Protoc (2016): se name="My EndNote Library.enl" path="C:\Use

Actin-Dynamics in Plant Cells: The Function of Actin-Perturbing Substances: Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins

Authors:

Holzinger, A., Blaas, K.

Journal:

Methods Mol Biol (2016): 243-61

Impact of C24:0 on actin-microtubule interaction in human neuronal SK-N-BE cells: evaluation by FRET confocal spectral imaging microscopy after dual staining with rhodamine-phalloidin and tubulin tracker green

Authors:

Zarrouk, A., Nury, T., Dauphin, A., Frere, P., Riedinger, J. M., Bachelet, C. M., Frouin, F., Moreau, T., Hammami, M., Kahn, E., Lizard, G.

Journal:

Funct Neurol (2015): 33-46

Protection against phalloidin-induced liver injury by oleanolic acid involves Nrf2 activation and suppression of Oatp1b2

Authors:

Lu, Y. F., Liu, J., Wu, K. C., Klaassen, C. D.

Journal:

Toxicol Lett (2015): 326-32

CLSM Analysis of the Phalloidin-Stained Muscle System of the Nemertean Proboscis and Rhynchocoel

Authors:

Chernyshev, A. V.

Journal:

Zoolog Sci (2015): 547-60

References

View all 151 references: Citation Explorer

DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes

Authors:

Sun, Ming-Hong and Yang, Mo and Xie, Feng-Yun and Wang, Wei and Zhang, Lili and Shen, Wei and Yin, Shen and Ma, Jun-Yu

Journal:

PloS one (2017): e0170308

Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression

Authors:

Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue

Journal:

Journal of Biosciences and Medicines (2017): 8

Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery

Authors:

Chang, Ting-Ya and Chen, Chen and Lee, Min and Chang, Ya-Chu and Lu, Chi-Huan and Lu, Shao-Tzu and Wang, De-Yao and Wang, Aijun and Guo, Chin-Lin and Cheng, Pei-Lin

Journal:

eLife (2017): e31101

Enhanced bovine serum albumin absorption on the N-hydroxysuccinimide activated graphene oxide and its corresponding cell affinity

Authors:

Xiong, Kun and Fan, Qingbo and Wu, Tingting and Shi, Haishan and Chen, Lin and Yan, Minhao

Journal:

Materials Science and Engineering: C (2017)

Cell-Permeable, MMP-2 Activatable, Nickel Ferrite and His-tagged Fusion Protein Self-Assembled Fluorescent Nanoprobe for Tumor Magnetic Targeting and Imaging

Authors:

Sun, Lu and Xie, Shuping and Qi, Jing and Liu, Ergang and Liu, Di and Liu, Quan and Chen, Sunhui and He, Huining and Yang, Victor C

Journal:

ACS Applied Materials & Interfaces (2017)

Physical properties

Molecular weight	1366.55
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on September 29, 2025