Name: Phen Green™ SK, Diacetate
Brand: AAT Bioquest
SKU: 21264
Price: 295 USD
Availability: InStock

Phen Green™ SK, Diacetate

Phen Green™ SK Diacetate is a cell-permeable fluorogenic chelator that detects multiple transition and heavy metal ions through fluorescence quenching, enabling real-time monitoring of intracellular metal dynamics.

Metal Ion Quenching Sensor: Fluorescence decreases upon binding Fe²⁺, Fe³⁺, Cu²⁺, and other transition metals
Live-Cell Permeability: Diacetate form enables dynamic monitoring of labile intracellular metal pools
Quantitative Detection: Concentration-dependent quenching allows measurement of metal ion levels
Calcein Alternative: Higher selectivity for transition metals versus calcium-sensitive indicators

The chemical structure of Phen Green™ SK, diacetate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21264	1 mg	Price

Overview

Phen Green™ SK, Diacetate is a cell-permeable, fluorogenic chelator designed for the sensitive detection of intracellular transition and heavy metal ions in live-cell applications. Upon cellular uptake, cytosolic esterases efficiently hydrolyze the diacetate protecting groups, yielding the highly fluorescent, metal-sensitive Phen Green™ SK dye. The activated probe exhibits excitation and emission maxima at approximately 507 nm and 532 nm, respectively, and undergoes fluorescence quenching in the presence of a broad range of divalent and monovalent metal ions, including Fe²⁺, Cu²⁺, Cu⁺, Zn²⁺, Cd²⁺, Ni²⁺, Co²⁺, Hg²⁺, and Pb²⁺.

This metal-induced quenching mechanism allows for direct, concentration-dependent monitoring of labile metal pools within the cytosol. Phen Green™ SK, Diacetate is well-suited for use in both fluorescence microscopy and high-throughput microplate assays, offering flexibility across experimental platforms. Its broad metal ion reactivity and robust signal response make it a powerful tool for investigating intracellular metal ion dynamics, redox regulation, metal transport mechanisms, and metal-associated cytotoxicity in diverse biological systems.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Phen Green™ SK, Diacetate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	141.545 µL	707.724 µL	1.415 mL	7.077 mL	14.154 mL
5 mM	28.309 µL	141.545 µL	283.09 µL	1.415 mL	2.831 mL
10 mM	14.154 µL	70.772 µL	141.545 µL	707.724 µL	1.415 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	706.49 g/mol	=	mL	x	mM	=	mmol

References

View all 41 references: Citation Explorer

Use of confocal laser scanning microscopy to evaluate the metal ion removal and destabilization of Enterococcus faecalis biofilms by EDTA and etidronic acid.

Authors:

Tartari, Talita and Estrela, Carlos and de Araújo, Larissa Barbosa Borges and Graeff, Márcia Sirlene Zardin and de Andrade, Flaviana Bombarda and Duarte, Marco Antonio Hungaro

Journal:

Odontology (2025)

Molecular characterization of STEAP3 in lung squamous cell carcinoma: Regulating EGFR to affect cell proliferation and ferroptosis.

Authors:

Shi, Hanqiang and Lei, Siyu and Xiong, Lie and Du, Shuqin and Shi, Yanbo

Journal:

Archives of biochemistry and biophysics (2024): 109842

Total extracts from Abelmoschus manihot (L.) alleviate radiation-induced cardiomyocyte ferroptosis via regulating redox imbalances mediated by the NOX4/xCT/GPX4 axis.

Authors:

Xu, Zhongchi and Wang, Yupeng and Yang, Wenli and Han, Weiyu and Ma, Beiting and Zhao, Yunhe and Bao, Tong and Zhang, Qi and Lin, Xin

Journal:

Journal of ethnopharmacology (2024): 118582

International consensus guidelines for the definition, detection, and interpretation of autophagy-dependent ferroptosis.

Authors:

Chen, Xin and Tsvetkov, Andrey S and Shen, Han-Ming and Isidoro, Ciro and Ktistakis, Nicholas T and Linkermann, Andreas and Koopman, Werner J H and Simon, Hans-Uwe and Galluzzi, Lorenzo and Luo, Shouqing and Xu, Daqian and Gu, Wei and Peulen, Olivier and Cai, Qian and Rubinsztein, David C and Chi, Jen-Tsan and Zhang, Donna D and Li, Changfeng and Toyokuni, Shinya and Liu, Jinbao and Roh, Jong-Lyel and Dai, Enyong and Juhasz, Gabor and Liu, Wei and Zhang, Jianhua and Yang, Minghua and Liu, Jiao and Zhu, Ling-Qiang and Zou, Weiping and Piacentini, Mauro and Ding, Wen-Xing and Yue, Zhenyu and Xie, Yangchun and Petersen, Morten and Gewirtz, David A and Mandell, Michael A and Chu, Charleen T and Sinha, Debasish and Eftekharpour, Eftekhar and Zhivotovsky, Boris and Besteiro, Sébastien and Gabrilovich, Dmitry I and Kim, Do-Hyung and Kagan, Valerian E and Bayir, Hülya and Chen, Guang-Chao and Ayton, Scott and Lünemann, Jan D and Komatsu, Masaaki and Krautwald, Stefan and Loos, Ben and Baehrecke, Eric H and Wang, Jiayi and Lane, Jon D and Sadoshima, Junichi and Yang, Wan Seok and Gao, Minghui and Münz, Christian and Thumm, Michael and Kampmann, Martin and Yu, Di and Lipinski, Marta M and Jones, Jace W and Jiang, Xuejun and Zeh, Herbert J and Kang, Rui and Klionsky, Daniel J and Kroemer, Guido and Tang, Daolin

Journal:

Autophagy (2024): 1213-1246

Biomineralisation and metal sequestration in a crustacean ectoparasite infecting the gills of a freshwater fish.

Authors:

Latief, Lutfiyya and Gilbert, Beric M and Avenant-Oldewage, Annemariè

Journal:

Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2023): 271-279

Physical properties

Molecular weight	706.49
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	234075-45-7

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 31, 2025