PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color*

PhosphoWorks™ Colorimetric Phosphate Assay Kit

Blue Color

PhosphoWorks™ Colorimetric Phosphate Assay Kit *Blue Color* is a colorimetric assay kit for quantifying inorganic phosphate (Pi) and measuring Pi-generating enzyme activity.

Detection Principle: Uses a malachite green derivative with molybdate for Pi detection.
Signal Advantage: Provides a stable endpoint signal with minimal precipitation.
Workflow: Develops color within 10–40 minutes.
Plate Format: Supports 96- or 384-well assays.
Instrument Compatibility: Absorbance detection at 600–660 nm using clear-bottom plates.

Phosphate dose response was measured with PhosphoWorks™ Colorimetric Phosphate Assay Kit on a clear 96-well plate using a SpectraMax Plus microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
21665	1000 Tests	Price

Overview

Phosphate is involved in many biological reactions. For example, phosphatases, ATPases and several other enzymes catalyze reactions in which inorganic phosphate (Pi) is released from a substrate. This PhosphoWorks™ Phosphate Assay Kit has been developed for measuring the activity of any Pi-generating enzyme. The kit is formulated to give sensitive detection of Pi, providing an alternative to hazardous radioactive methods and other less sensitive colorimetric assays. The measurement of Pi is based on the change in absorbance of a malachite green derivative in the presence of molybdate. Unlike other malachite dye formulations, this kit gives a completely stable end-point signal that is not prone to precipitation.

Citations

View all 9 citations: Citation Explorer

Variation of the bone forming ability with the physicochemical properties of calcium phosphate bone substitutes

Authors:

Duan, Rongquan and Barbieri, Davide and Luo, Xiaoman and Weng, Jie and Bao, Chongyun and De Bruijn, Joost D and Yuan, Huipin

Journal:

Biomaterials science (2018): 136--145

Modulating bone regeneration in rabbit condyle defects with three surface-structured tricalcium phosphate ceramics

Authors:

Duan, Rongquan and Barbieri, Davide and de. groot, Florence and de. bruijn, Joost and Yuan, Huipin

Journal:

ACS Biomaterials Science & Engineering (2018)

Variation of bone forming ability with the physicochemical properties of calcium phosphate bone substitutes

Authors:

Duan, Rongquan and Barbieri, Davide and Luo, Xiaoman and Weng, Jie and Bao, Chongyun and De Bruijn, Joost and Yuan, Huipin

Journal:

Biomaterials Science (2017)

Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs.

Authors:

Davison, NL and Su, J and Yuan, Huipin and Van Den Beucken, JJJP and de Bruijn, Joost Dick and others,

Influence of surface microstructure and chemistry on osteoinduction and osteoclastogenesis by biphasic calcium phosphate discs.

Authors:

Davison, NL and Su, J and Yuan, H and van den Beucken, JJJP and de Bruijn, JD and others, undefined

References

View all 16 references: Citation Explorer

Cycling of NADPH by glucose 6-phosphate dehydrogenase optimizes the spectrophotometric assay of nitric oxide synthase activity in cell lysates

Authors:

Ghigo D, Riganti C, Gazzano E, Costamagna C, Bosia A.

Journal:

Nitric Oxide (2006): 148

Tartrate-resistant acid phosphate activity as osteoclastic marker: sensitivity of cytochemical assessment and serum assay in comparison with standardized osteoclast histomorphometry

Authors:

Ballanti P, Minisola S, Pacitti MT, Scarnecchia L, Rosso R, Mazzuoli GF, Bonucci E.

Journal:

Osteoporos Int (1997): 39

Continuous assay for acid phosphatase using phenyl phosphate

Authors:

Luchter-Wasylewska E., undefined

Journal:

Anal Biochem (1996): 167

A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems

Authors:

Webb MR., undefined

Journal:

Proc Natl Acad Sci U S A (1992): 4884

Detection of glucose-6-phosphate dehydrogenase-deficient heterozygotes: cytochemical method and spectrophotometric assay

Authors:

Renner H, Michel P, Solem E.

Journal:

Clin Chim Acta (1988): 239

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Spectrophotometer
Absorbance	600 - 660 nm
Recommended plate	Clear bottom

Absorbance microplate reader
Absorbance	600 - 660 nm
Recommended plate	Clear bottom

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Page updated on October 28, 2026

Component A: 1 mM KH2PO4 Standard	1 vial (1 mL)
Component B: MG Plus™ Reagent	1 bottle (20 mL)