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PhosphoWorks™ Luminometric ATP Assay Kit
DTT-Free
Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks™ ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit does not use DTT, and has the stable luminescence signal as long as 4 hours. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.
ATP dose response was measured with the PhosphoWorks™ Luminescence ATP Assay Kit *DTT-Free* on a 96-well white plate using a NOVOstar plate reader (BMG Labtech). The kit can detect (3 pmole/well) 0.03 nM ATP within 20 min. The integration time was 1 sec. The half life is more than 2 hours.
ATP dose response was measured with the PhosphoWorks™ Luminescence ATP Assay Kit *DTT-Free* on a 96-well white plate using a NOVOstar plate reader (BMG Labtech). The kit can detect (3 pmole/well) 0.03 nM ATP within 20 min. The integration time was 1 sec. The half life is more than 2 hours.
protocol
Protocol
COA
CatalogSize
Price
Quantity
216121 Plate
Price
2161310 Plates
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Luminescence microplate reader
Recommended plateSolid white
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Page updated on September 25, 2025