Name: PhosphoWorks™ Luminometric ATP Assay Kit *DTT-Free*
Brand: AAT Bioquest
SKU: 21612
Availability: InStock

PhosphoWorks™ Luminometric ATP Assay Kit

DTT-Free

PhosphoWorks™ Luminometric ATP Assay Kit *DTT-Free* is a luminometric assay kit for quantifying ATP and assessing cell proliferation and cytotoxicity in mammalian cells.

Detection Principle: Uses firefly luciferase to detect ATP by luminescence.
Sensitivity: Detects ATP down to 0.03 nM within 20 minutes.
Signal Advantage: Provides stable luminescence for up to 4 hours.
Workflow: DTT-free assay with no mixing or separation required.
Plate Format: Supports 96- or 384-well assays.

ATP dose response was measured with the PhosphoWorks™ Luminescence ATP Assay Kit *DTT-Free* on a 96-well white plate using a NOVOstar plate reader (BMG Labtech). The kit can detect (3 pmole/well) 0.03 nM ATP within 20 min. The integration time was 1 sec. The half life is more than 2 hours.

Protocol

Catalog	Size	Price	Quantity
21612	1 Plate	Price
21613	10 Plates	Price

Overview

Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling. The PhosphoWorks™ ATP Assay Kit provides a fast, simple and homogeneous luminescence assay for the determination of cell proliferation and cytotoxicity in mammalian cells. The assay can be performed in a convenient 96-well and 384-well microtiter-plate format. The high sensitivity of this assay permits the detection of ATP in many biological systems, environmental samples and foods. This PhosphoWorks ATP Assay Kit does not use DTT, and has the stable luminescence signal as long as 4 hours. It has stable luminescence with no mixing or separations required, and formulated to have minimal hands-on time.

Citations

View all 13 citations: Citation Explorer

ACSL4 Drives C5a/C5aR1--Calcium-Induced Fibroblast-to-Myofibroblast Transition in a Bleomycin-Induced Mouse Model of Pulmonary Fibrosis

Authors:

Ren, Tingting and Shi, Jia and Zhuang, Lili and Su, Ruiting and Lai, Yimei and Yang, Niansheng

Journal:

Biomolecules (2025): 1106

Palmitic Acid Exerts Anti-Tumorigenic Activities by Modulating Cellular Stress and Lipid Droplet Formation in Endometrial Cancer

Authors:

Zhao, Ziyi and Wang, Jiandong and Kong, Weimin and Newton, Meredith A and Burkett, Wesley C and Sun, Wenchuan and Buckingham, Lindsey and O’Donnell, Jillian and Suo, Hongyan and Deng, Boer and others,

Journal:

Biomolecules (2024): 601

Knocking out Fkbp51 decreases CCl4-induced liver injury through enhancement of mitochondrial function and Parkin activity

Authors:

Qiu, Bin and Zhong, Zhaohui and Dou, Longyu and Xu, Yuxue and Zou, Yi and Weldon, Korri and Wang, Jun and Zhang, Lingling and Liu, Ming and Williams, Kent E and others,

Journal:

Cell \& Bioscience (2024): 1

The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells

Authors:

Chen, Ya-Hui and Chen, Yi-Chun and Liu, Chin-San and Hsieh, Ming-Chia

Journal:

Journal of Diabetes Research (2016)

NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo

Authors:

Zhang, Lu and Han, Jianjun and Jackson, Am and a L , undefined and Clark, Leslie N and Kilgore, Joshua and Guo, Hui and Livingston, Nick and Batchelor, Kenneth and Yin, Yajie and Gilliam, Timothy P and others, undefined

Journal:

Journal of Hematology & Oncology (2016): 91

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Luminescence microplate reader
Recommended plate	Solid white

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Page updated on October 28, 2026

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor (Light-sensitive)	1 vial
Component C: Reaction Buffer	1 vial (10 mL)

Component A: ATP Monitoring Enzyme	1 vial
Component B: ATP Sensor	10 vials
Component C: Reaction Buffer	2 vials (50 mL/vial)