pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4*

pNPP

4-Nitrophenyl phosphate, disodium salt; CAS 333338-18-4

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. In the presence of phosphatases pNPP is hydrolyzed to generate the yellow para-nitrophenol that can be measured at 405 nm with an absorption microplate reader or a spectrophotometer.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11619	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	269.44 µL	1.347 mL	2.694 mL	13.472 mL	26.944 mL
5 mM	53.888 µL	269.44 µL	538.88 µL	2.694 mL	5.389 mL
10 mM	26.944 µL	134.72 µL	269.44 µL	1.347 mL	2.694 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	371.14 g/mol	=	mL	x	mM	=	mmol

References

View all 52 references: Citation Explorer

Reference Interval of Serum Heat-stable Alkaline Phosphatase Activity in Pregnant Women in Zaria

Authors:

Aliyu IS, Isah HS, Afonja OA.

Journal:

Niger Postgrad Med J (2006): 31

Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a

Authors:

Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.

Journal:

Clin Chim Acta (2005): 132

Alternative immunoassay for tartrate-resistant acid phosphatase isoform 5b using the fluorogenic substrate naphthol ASBI-phosphate and heparin

Authors:

Janckila AJ, Simons RM, Yam LT.

Journal:

Clin Chim Acta (2004): 157

The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase

Authors:

Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined

Journal:

Proc Natl Acad Sci U S A (2003): 1552

Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization

Authors:

Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.

Journal:

Folia Microbiol (Praha) (2003): 627

Physical properties

Molecular weight	371.14
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	333338-18-4

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Page updated on October 9, 2025