pNPP [4-Nitrophenyl phosphate, disodium salt] CAS 333338-18-4

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Physical properties

Molecular weight	371.14
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

333338-18-4

Molecular weight

371.14

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. In the presence of phosphatases pNPP is hydrolyzed to generate the yellow para-nitrophenol that can be measured at 405 nm with an absorption microplate reader or a spectrophotometer.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	269.44 µL	1.347 mL	2.694 mL	13.472 mL	26.944 mL
5 mM	53.888 µL	269.44 µL	538.88 µL	2.694 mL	5.389 mL
10 mM	26.944 µL	134.72 µL	269.44 µL	1.347 mL	2.694 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4*

References

View all 52 references: Citation Explorer

Reference Interval of Serum Heat-stable Alkaline Phosphatase Activity in Pregnant Women in Zaria
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31

Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132

Alternative immunoassay for tartrate-resistant acid phosphatase isoform 5b using the fluorogenic substrate naphthol ASBI-phosphate and heparin
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157

The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552

Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627

Chemiluminescent assay of alkaline phosphatase using dihydroxyacetone phosphate as substrate detected with lucigenin
Authors: Kokado A, Arakawa H, Maeda M.
Journal: Luminescence (2002): 5

A heat-sensitive Arabidopsis thaliana kinase substitutes for human p70s6k function in vivo
Authors: Turck F, Kozma SC, Thomas G, Nagy F.
Journal: Mol Cell Biol (1998): 2038

Partially folded conformations of inositol monophosphatase endowed with catalytic activity
Authors: Lau CK, Lo SC, Li W, Churchich DR, Kwok F, Churchich JE.
Journal: J Protein Chem (1998): 789

Topological studies on the enzymes catalyzing the biosynthesis of Glc-P-dolichol and the triglucosyl cap of Glc3Man9GlcNAc2-P-P-dolichol in microsomal vesicles from pig brain: use of the processing glucosidases I/II as latency markers
Authors: Rush JS, Waechter CJ.
Journal: Glycobiology (1998): 1207

Solvent kinetic isotope effects of human placental alkaline phosphatase in reverse micelles
Authors: Huang TM, Hung HC, Chang TC, Chang GG.
Journal: Biochem J (1998): 267