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pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4*

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA assays. In the presence of phosphatases pNPP is hydrolyzed to generate the yellow para-nitrophenol that can be measured at 405 nm with an absorption microplate reader or a spectrophotometer.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of pNPP [4-Nitrophenyl phosphate, disodium salt] *CAS 333338-18-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM269.44 µL1.347 mL2.694 mL13.472 mL26.944 mL
5 mM53.888 µL269.44 µL538.88 µL2.694 mL5.389 mL
10 mM26.944 µL134.72 µL269.44 µL1.347 mL2.694 mL

Molarity calculator

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References

View all 52 references: Citation Explorer
Reference Interval of Serum Heat-stable Alkaline Phosphatase Activity in Pregnant Women in Zaria
Authors: Aliyu IS, Isah HS, Afonja OA.
Journal: Niger Postgrad Med J (2006): 31
Development of immunoassays for serum tartrate-resistant acid phosphatase isoform 5a
Authors: Chao TY, Lee SH, Chen MM, Neustadt DH, Chaudhry UA, Yam LT, Janckila AJ.
Journal: Clin Chim Acta (2005): 132
Alternative immunoassay for tartrate-resistant acid phosphatase isoform 5b using the fluorogenic substrate naphthol ASBI-phosphate and heparin
Authors: Janckila AJ, Simons RM, Yam LT.
Journal: Clin Chim Acta (2004): 157
The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase
Authors: Cronin A, Mowbray S, Durk H, Homburg S, Fleming I, Fisslthaler B, Oesch F, Ar and M., undefined
Journal: Proc Natl Acad Sci U S A (2003): 1552
Extracellular alkaline phosphatase from the filamentous fungus Aspergillus caespitosus: purification and biochemical characterization
Authors: Guimaraes LH, Terenzi HF, Jorge JA, Leone FA, Polizeli ML.
Journal: Folia Microbiol (Praha) (2003): 627
Page updated on October 12, 2024

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Physical properties

Molecular weight

371.14

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

333338-18-4
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