Portelite™ Fluorimetric RNA Quantitation Kit*Optimized for Cytocite™ and Qubit™ Fluorometers*

Portelite™ Fluorimetric RNA Quantitation Kit

Optimized for Cytocite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric RNA Quantitation Kit is a fluorimetric assay kit for quantifying RNA in solution.

Detection Principle: StrandBrite™ Green fluoresces upon binding RNA.
Sensitivity: Detects RNA down to 5 ng/mL.
Workflow: 2-minute room-temperature incubation.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers.
Detection Settings: Ex/Em 480/530 nm using 0.2 mL PCR tubes.

RNA standard curve generated using StrandBrite™ Green Fluorimetric RNA Quantitation Kit. Fluorescence intensity was quantified using green fluorescence channel; regression model was calculated using linear fit.

Catalog	Size	Price	Quantity
17658	100 Tests	Price
17659	500 Tests	Price

Overview

Detecting and quantitating small amounts of RNA is extremely important for a wide variety of biological applications such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm. The absorbance-based method is limited by the interferences caused from proteins, free nucleotides and other UV absorbing compounds. The use of sensitive and selective fluorescent nucleic acid stains alleviates this interference problem. StrandBrite™ RNA quantifying reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. StrandBrite™ RNA quantifying reagent can detect as little as 5 ng/mL RNA with a CytoCite™ or Qubit™ fluorometer. Our StrandBrite™ Green Fluorimetric RNA Quantitation Kit includes our StrandBrite™ Green nucleic acid stain with an optimized and robust protocol. It provides a convenient method for quantifying RNA in solutions.

References

View all 50 references: Citation Explorer

Oncologic Implications of Chronic Hepatitis C Virus Infection.

Authors:

Hwang, Jessica P and LoConte, Noelle K and Rice, John P and Foxhall, Lewis E and Sturgis, Erich M and Merrill, Janette K and Torres, Harrys A and Bailey, Howard H

Journal:

Journal of oncology practice (2019): 629-637

Outcomes and costs of single-step hepatitis C testing in primary care, Birmingham, United Kingdom.

Authors:

Munang, M and Smit, E and Barnett, T and Atherton, C and Tahir, M and Atabani, S F

Journal:

Public health (2019): 40-44

Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots.

Authors:

Weber, Jenna and Sahoo, Malaya K and Taylor, Nathaniel and Shi, Run-Zhang and Pinsky, Benjamin A

Journal:

Journal of clinical microbiology (2019)

PARP-1/2 Inhibitor Olaparib Prevents or Partially Reverts EMT Induced by TGF-β in NMuMG Cells.

Authors:

Schacke, Michelle and Kumar, Janani and Colwell, Nicholas and Hermanson, Kole and Folle, Gustavo A and Nechaev, Sergei and Dhasarathy, Archana and Lafon-Hughes, Laura

Journal:

International journal of molecular sciences (2019)

Tumor-draining lymph nodes demonstrate a suppressive immunophenotype in patients with non-small cell lung cancer assessed by endobronchial ultrasound-guided transbronchial needle aspiration: A pilot study.

Authors:

Murthy, Vivek and Katzman, Daniel P and Tsay, Jun-Chieh J and Bessich, Jamie L and Michaud, Gaetane C and Rafeq, Samaan and Minehart, Janna and Mangalick, Keshav and de Lafaille, M A Curotto and Goparaju, Chandra and Pass, Harvey and Sterman, Daniel H

Journal:

Lung cancer (Amsterdam, Netherlands) (2019): 94-99

Spectral properties

Excitation (nm)	509
Emission (nm)	527

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

CytoCite Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

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Page updated on October 23, 2026

Component A: StrandBrite™ Green	0.25 mL (200X in DMSO)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: RNA Standard #1	1 mL (RNA: 0 ng/μL)
Component D: RNA Standard #2	4 X 250 μL (RNA: 10 ng/μL)

Component A: StrandBrite™ Green	1.25 mL (200X in DMSO)
Component B: Assay Buffer	250 mL
Component C: RNA Standard #1	5 mL (RNA: 0 ng/μL)
Component D: RNA Standard #2	10 X 500 μL (RNA: 10 ng/μL)