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Portelite™ Fluorimetric ssDNA Quantitation Kit
Optimized for Cytocite™ and Qubit™ Fluorometers
Portelite™ Fluorimetric ssDNA Quantitation Kit is designed to rapidly measure single-stranded DNA. The kit has all the essential reagents, including Helixyte™ Green ssDNA reagent, dilution buffer, and prediluted DNA standards. Helixyte™ Green ssDNA reagent is a sensitive fluorescent nucleic acid probe for quantifying oligonucleotides and single-stranded DNA (ssDNA) in solution. Simply dilute the reagent using the buffer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using CytoCite, Qubit® or other hand-held or desk top fluorometers (Qubit® is the trademark of ThermoFisher). The assay is accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL, providing an assay range of 1–200 ng. The assay detects long oligonucleotides or ssDNA. Nucleotides and short oligo nucleotides of six bases or less do not interfere in the quantitation assay. The detection limit is not significantly interfered by the common contaminants in nucleic acid preparations, including salts, urea, ethanol, chloroform, detergents, proteins, nucleotides and short oligonucleotides of six bases. However, double-stranded DNA (dsDNA) and RNA do interfere with the assay as Helixyte™ Green ssDNA reagent binds to dsDNA and RNA to generate additional fluorescence signal. Portelite™ Fluorimetric ssDNA Quantitation Kit is optimized for CytoCite™ and Qubit® fluorometers.
Comparison of ssDNA dose response using the Qubit™ fluorometer (blue) or CytoCite™ fluorometer (red).
Comparison of ssDNA dose response using the Qubit™ fluorometer (blue) or CytoCite™ fluorometer (red).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
17625200 Tests
Price
 
Spectral properties
Excitation (nm)498
Emission (nm)519
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Qubit Fluorometer
Excitation480 nm
Emission530 nm
Instrument specification(s)0.2 mL PCR vial
CytoCite Fluorometer
Excitation480 nm
Emission530 nm
Instrument specification(s)0.2 mL PCR vial
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Page updated on October 13, 2025