Name: Portelite™ Fluorimetric ssDNA Quantitation Kit *Optimized for Cytocite™ and Qubit™ Fluorometers*
Brand: AAT Bioquest
SKU: 17625
Price: 329 USD
Availability: InStock

Portelite™ Fluorimetric ssDNA Quantitation Kit

Optimized for Cytocite™ and Qubit™ Fluorometers

Portelite™ Fluorimetric ssDNA Quantitation Kit is a fluorimetric assay kit for quantifying single-stranded DNA and long oligonucleotides in solution.

Detection Principle: Helixyte™ Green ssDNA fluoresces upon nucleic acid binding.
Quantitation Range: Measures 1–200 ng ssDNA per assay.
Selectivity: Detects ssDNA and long oligonucleotides; short oligos ≤6 bases do not interfere.
Workflow: 2-minute room-temperature incubation before reading.
Instrument Compatibility: Optimized for CytoCite™ and Qubit™ fluorometers at Ex/Em 480/530 nm.

Comparison of ssDNA dose response using the Qubit™ fluorometer (blue) or CytoCite™ fluorometer (red).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17625	200 Tests	Price

Overview

Portelite™ Fluorimetric ssDNA Quantitation Kit is designed to rapidly measure single-stranded DNA. The kit has all the essential reagents, including Helixyte™ Green ssDNA reagent, dilution buffer, and prediluted DNA standards. Helixyte™ Green ssDNA reagent is a sensitive fluorescent nucleic acid probe for quantifying oligonucleotides and single-stranded DNA (ssDNA) in solution. Simply dilute the reagent using the buffer provided, add your sample (any volume from 1–20 μL is acceptable), then read the concentration using CytoCite, Qubit® or other hand-held or desk top fluorometers (Qubit® is the trademark of ThermoFisher). The assay is accurate for initial sample concentrations from 50 pg/µL to 200 ng/µL, providing an assay range of 1–200 ng. The assay detects long oligonucleotides or ssDNA. Nucleotides and short oligo nucleotides of six bases or less do not interfere in the quantitation assay. The detection limit is not significantly interfered by the common contaminants in nucleic acid preparations, including salts, urea, ethanol, chloroform, detergents, proteins, nucleotides and short oligonucleotides of six bases. However, double-stranded DNA (dsDNA) and RNA do interfere with the assay as Helixyte™ Green ssDNA reagent binds to dsDNA and RNA to generate additional fluorescence signal. Portelite™ Fluorimetric ssDNA Quantitation Kit is optimized for CytoCite™ and Qubit® fluorometers.

References

View all 24 references: Citation Explorer

An OliGreen-responsive fluorescence sensor for sensitive detection of organophosphorus pesticide based on its specific selectivity towards T-Hg2+-T DNA structure.

Authors:

Zhou, Xiaoyuan and Wang, Chenchen and Wu, Lina and Wei, Wei and Liu, Songqin

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2021): 119155

A novel detection of radon based on its decay product inducing conformational changes of an aptamer probe.

Authors:

Long, Minzhi and Deng, Han and Tian, Gang and Song, Chunli and Liu, Hongwen and Shen, Yi and Lv, Changyin

Journal:

Analytica chimica acta (2016): 202-7

Studies of DNA Aptamer OliGreen and PicoGreen Fluorescence Interactions in Buffer and Serum.

Authors:

Bruno, John G and Sivils, Jeffrey C

Journal:

Journal of fluorescence (2016): 1479-87

Accumulation of single-stranded DNA in Escherichia coli carrying the colicin plasmid pColE3-CA38.

Authors:

Morales, Magali and Attai, Hedieh and Troy, Kimberly and Bermudes, David

Journal:

Plasmid (2015): 7-16

Probing the inherent stability of siRNA immobilized on nanoparticle constructs.

Authors:

Barnaby, Stacey N and Lee, Andrew and Mirkin, Chad A

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2014): 9739-44

Spectral properties

Excitation (nm)	498
Emission (nm)	519

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Qubit Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

CytoCite Fluorometer
Excitation	480 nm
Emission	530 nm
Instrument specification(s)	0.2 mL PCR vial

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Shipping	Standard overnight for United States, inquire for international

Page updated on November 15, 2026

Component A: Helixyte™ Green ssDNA	1 vial (0.25 mL-200X DMSO)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: ssDNA Standard #1	1 vial (1 mL: 0 ng/µL)
Component D: ssDNA Standard #2	1 vial (1 mL, 10 ng/µL)