Pro-AMC | AAT Bioquest

Pro-AMC

Pro-AMC is a sensitive fluorogenic protease substrate. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of tricorn protease (TRI), a high molecular mass complex from the archaeon T. acidophilum.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13457	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Pro-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	367.242 µL	1.836 mL	3.672 mL	18.362 mL	36.724 mL
5 mM	73.448 µL	367.242 µL	734.484 µL	3.672 mL	7.345 mL
10 mM	36.724 µL	183.621 µL	367.242 µL	1.836 mL	3.672 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	272.3 g/mol	=	mL	x	mM	=	mmol

References

View all 79 references: Citation Explorer

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease

Authors:

Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.

Journal:

Anal Biochem (2009): 195

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model

Authors:

Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.

Journal:

Gynecol Oncol (2009): 616

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7

Authors:

Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.

Journal:

J Biomol Screen (2009): 1

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates

Authors:

Nakata H, Ohtsuki T, Sisido M.

Journal:

Anal Biochem (2009): 121

Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes

Authors:

An L, Liu L, Wang S.

Journal:

Biomacromolecules (2009): 454

Physical properties

Molecular weight	272.30
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025