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PRO-TAO-3 [equivalent to TO-PRO-3]

Chemical structure for PRO-TAO-3 [equivalent to TO-PRO-3].
Chemical structure for PRO-TAO-3 [equivalent to TO-PRO-3].
Ordering information
Price ()Discontinued
Catalog Number17565
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight671.42
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)102000
Excitation (nm)642
Emission (nm)657
Quantum yield0.111
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
157199-63-8
Molecular weight
671.42
Extinction coefficient (cm -1 M -1)
102000
Excitation (nm)
642
Emission (nm)
657
Quantum yield
0.111
PRO-TAO-3 is the same molecule to TO-PRO®-3 stain. TO-PRO®-3 and the other monomeric cyanine stains have wide applicability due to their low background and bright fluorescence. It is a carbocyanine monomer nucleic acid stain that can be well excited by a red laser at 633 nm or 647 nm with far-red fluorescence similar to Cy®5 dyes. It is useful as a nuclear counterstain and dead cell indicator. It is selective for detecting dsDNA (double-stranded nucleic acids). The cell-impermeable PRO-TTO-3 is widely used for flow cytometry and fluorescence microscopy as a long-wavelength fluorescence well separated from green and red fluorophores in multicolor applications. TO-PRO® is the trademark of ThermoFisher Scientific.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of PRO-TAO-3 [equivalent to TO-PRO-3] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM148.938 µL744.69 µL1.489 mL7.447 mL14.894 mL
5 mM29.788 µL148.938 µL297.876 µL1.489 mL2.979 mL
10 mM14.894 µL74.469 µL148.938 µL744.69 µL1.489 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)102000
Excitation (nm)642
Emission (nm)657
Quantum yield0.111

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
TWO-PRO™ 3 [equivalent to TO-PRO®-3] *5 mM DMSO Solution* *CAS 157199-63-8*64265710200010.111

References


View all 50 references: Citation Explorer
Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.
Authors: Escoter-Torres, Laura and Greulich, Franziska and Quagliarini, Fabiana and Wierer, Michael and Uhlenhaut, Nina Henriette
Journal: Nucleic acids research (2020)
Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction.
Authors: Clementi, Elena and Inglin, Larissa and Beebe, Erin and Gsell, Corina and Garajova, Zuzana and Markkanen, Enni
Journal: BMC biology (2020): 36
DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.
Authors: Kyono, Yasuhiro and Raj, Samhitha and Sifuentes, Christopher J and Buisine, Nicolas and Sachs, Laurent and Denver, Robert J
Journal: Developmental biology (2020): 180-196
Mithramycin selectively attenuates DNA-damage-induced neuronal cell death.
Authors: Makarevich, Oleg and Sabirzhanov, Boris and Aubrecht, Taryn G and Glaser, Ethan P and Polster, Brian M and Henry, Rebecca J and Faden, Alan I and Stoica, Bogdan A
Journal: Cell death & disease (2020): 587
DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.
Authors: Shao, Zhengping and Flynn, Ryan A and Crowe, Jennifer L and Zhu, Yimeng and Liang, Jialiang and Jiang, Wenxia and Aryan, Fardin and Aoude, Patrick and Bertozzi, Carolyn R and Estes, Verna M and Lee, Brian J and Bhagat, Govind and Zha, Shan and Calo, Eliezer
Journal: Nature (2020): 291-296
Acute aluminum chloride toxicity revisited: Study on DNA damage and histopathological, biochemical and neurochemical alterations in rat brain.
Authors: Liaquat, Laraib and Sadir, Sadia and Batool, Zehra and Tabassum, Saiqa and Shahzad, Sidrah and Afzal, Asia and Haider, Saida
Journal: Life sciences (2019): 202-211
Selectivity of Terpyridine Platinum Anticancer Drugs for G-quadruplex DNA.
Authors: Morel, Elodie and Beauvineau, Claire and Naud-Martin, Delphine and Landras-Guetta, Corinne and Verga, Daniela and Ghosh, Deepanjan and Achelle, Sylvain and Mahuteau-Betzer, Florence and Bombard, Sophie and Teulade-Fichou, Marie-Paule
Journal: Molecules (Basel, Switzerland) (2019)
DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells.
Authors: Liu, Dongjie and Li, Rui and Guo, Xianghua and Pang, Lijun and Zang, Yunjin and Liu, Kai and Chen, Dexi
Journal: Oncology letters (2018): 9333-9338
Regular exercise participation improves genomic stability in diabetic patients: an exploratory study to analyse telomere length and DNA damage.
Authors: Dimauro, Ivan and Sgura, Antonella and Pittaluga, Monica and Magi, Fiorenza and Fantini, Cristina and Mancinelli, Rosa and Sgadari, Antonio and Fulle, Stefania and Caporossi, Daniela
Journal: Scientific reports (2017): 4137
TP53 R72P polymorphism modulates DNA methylation in hepatocellular carcinoma.
Authors: Rebbani, Khadija and Marchio, Agnès and Ezzikouri, Sayeh and Afifi, Rajaa and Kandil, Mostafa and Bahri, Olfa and Triki, Henda and El Feydi, Abdellah Essaid and Dejean, Anne and Benjelloun, Soumaya and Pineau, Pascal
Journal: Molecular cancer (2015): 74