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Probenecid
Cell culture tested; CAS 57-66-9
Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. Each vial is dissovled in 0.3 mL of 1 M NaOH, and then diluted into 100 mL buffer to make 2.5 mM final concentration of probenecid for your assays.
Chemical structure for Probenecid *Cell culture tested* *CAS 57-66-9*
Chemical structure for Probenecid *Cell culture tested* *CAS 57-66-9*
CatalogSize
Price
Quantity
2006010x72 mg
Price
 
Physical properties

Molecular weight285.36
Solvent1 M NaOH
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C); Minimize light exposure
UNSPSC12352200
CAS57-66-9
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Page updated on October 9, 2025