Probenecid *Cell culture tested* *CAS 57-66-9*

Probenecid

Cell culture tested; CAS 57-66-9

Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. Each vial is dissovled in 0.3 mL of 1 M NaOH, and then diluted into 100 mL buffer to make 2.5 mM final concentration of probenecid for your assays.

Protocol

SDS

Catalog	Size	Price	Quantity
20060	10x72 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of 1 M NaOH needed to reconstitute specific mass of Probenecid *Cell culture tested* *CAS 57-66-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	350.435 µL	1.752 mL	3.504 mL	17.522 mL	35.043 mL
5 mM	70.087 µL	350.435 µL	700.869 µL	3.504 mL	7.009 mL
10 mM	35.043 µL	175.217 µL	350.435 µL	1.752 mL	3.504 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	285.36 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Magnetic-field-synchronized wireless modulation of neural activity by magnetoelectric nanoparticles

Authors:

Zhang, E and Abdel-Mottaleb, M and Liang, P and Navarrete, B and Yildirim, Y Akin and Campos, M Alberteris and Smith, IT and Wang, P and Yildirim, B and Yang, L and others,

Journal:

Brain Stimulation (2022): 1451--1462

Pharmacogenomic screening of drug candidates using patient-specific hiPSC-derived cardiomyocyte high-throughput calcium imaging

Authors:

Blancard, Malorie and Fetterman, K Ashley and Burridge, Paul W

Identification of the material basis of the medicinal properties in Curcuma Longa L. to enhance targeted clinical application

Authors:

Peng, Sha and Li, Jing and Huo, Mengqi and Cao, Yanfeng and Chen, Zhao and Zhang, Yanling and Qiao, Yanjiang

Journal:

Journal of Traditional Chinese Medical Sciences (2022): 374--382

Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling

Authors:

Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M

Journal:

Current protocols (2022): e330

A novel Ca2+ indicator for long-term tracking of intracellular calcium flux

Authors:

Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun

Journal:

BioTechniques (2021): 271--277

References

View all 35 references: Citation Explorer

DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833

Authors:

Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.

Journal:

Toxicol Lett (2007): 3

Is the monkey an appropriate animal model to examine drug-drug interactions involving renal clearance? Effect of probenecid on the renal elimination of H2 receptor antagonists

Authors:

Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.

Journal:

J Pharmacol Exp Ther (2006): 1187

Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid

Authors:

Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.

Journal:

Drug Metab Dispos (2006): 743

MRP2 (ABCC2) transports taxanes and confers paclitaxel resistance and both processes are stimulated by probenecid

Authors:

Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.

Journal:

Int J Cancer (2005): 824

Mediation of annexin 1 secretion by a probenecid-sensitive ABC-transporter in rat inflamed mucosa

Authors:

Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.

Journal:

Biochem Pharmacol (2004): 1195

Physical properties

Molecular weight	285.36
Solvent	1 M NaOH

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Room temperature (10-25 °C); Minimize light exposure
UNSPSC	12352200
CAS	57-66-9

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Calcium Detection Probes & Assay Kits
Brochure: Physiological Probes & Assay Kits

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Page updated on October 29, 2025