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Probenecid *Cell culture tested* *CAS 57-66-9*

Chemical structure for Probenecid *Cell culture tested* *CAS 57-66-9*
Chemical structure for Probenecid *Cell culture tested* *CAS 57-66-9*
Ordering information
Price ()
Catalog Number20060
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight285.36
Solvent1 M NaOH
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
57-66-9
Molecular weight
285.36
Probenecid is an inhibitor of organic-anion transporters located in cell membranes. These transporters often extrude fluorescent indicators from cells, and therefore contribute to poor dye retention. This phenomenon usually causes high background in the assays that require the good retention of the dye indicators inside cells. The use of probenecid to inhibit the transporter activity, and thus to reduce leakage of the intracellular dye indicators is a common method for reducing fluorescence background of calcium assays. Each vial is dissovled in 0.3 mL of 1 M NaOH, and then diluted into 100 mL buffer to make 2.5 mM final concentration of probenecid for your assays.

Calculators


Common stock solution preparation

Table 1. Volume of 1 M NaOH needed to reconstitute specific mass of Probenecid *Cell culture tested* *CAS 57-66-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM350.435 µL1.752 mL3.504 mL17.522 mL35.043 mL
5 mM70.087 µL350.435 µL700.869 µL3.504 mL7.009 mL
10 mM35.043 µL175.217 µL350.435 µL1.752 mL3.504 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Citations


View all 9 citations: Citation Explorer
Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling
Authors: Cambra, Hailey M and Tallapragada, Naren P and Mannam, Prabhath and Breault, David T and Klein, Allon M
Journal: Current protocols (2022): e330
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
Authors: Liao, Jinfang and Patel, Deven and Zhao, Qin and Peng, Ruogu and Guo, Haitao and Diwu, Zhenjun
Journal: BioTechniques (2021): 271--277
Electrophysiological Effects of Extracellular Vesicles Secreted by Cardiosphere-Derived Cells: Unraveling the Antiarrhythmic Properties of Cell Therapies
Authors: G{\'o}mez-Cid, Lidia and Moro-L{\'o}pez, Marina and de la Nava, Ana S and Hern{\'a}ndez-Romero, Ismael and Fern{\'a}ndez, Ana I and Su{\'a}rez-Sancho, Susana and Atienza, Felipe and Grigorian-Shamagian, Lilian and Fern{\'a}ndez-Avil{\'e}s, Francisco
Journal: Processes (2020): 924
Integrative Cardiovascular Physiology and Pathophysiology: Absence of gravin-mediated signaling inhibits development of high-fat diet-induced hyperlipidemia and atherosclerosis
Authors: Fan, Qiying and Yin, Xing and Rababa’h, Abeer and Diaz, Andrea Diaz and Wijaya, Cori S and Singh, Sonal and Suryavanshi, Santosh V and Vo, Henry Hiep and Saeed, Moawiz and Zhang, Yang and others,
Journal: American Journal of Physiology-Heart and Circulatory Physiology (2019): H793
Etablierung eines 3D in vitro Blutgef{\"a}{\ss}-/Gewebemodells zur Testung spezifischer Therapeutika zur Leuk{\"a}miebehandlung
Authors: Bersi, Heidi
Journal: (2017)
Teleocidin A2 inhibits human proteinase-activated receptor 2 signaling in tumor cells
Authors: Stahn, Sonja and Thelen, Lisa and Albrecht, Ina-Maria and Bitzer, Jens and Henkel, Thomas and Teusch, Nicole Elisabeth
Journal: Pharmacology Research & Perspectives (2016)
Glutathione adducts on sarcoplasmic/endoplasmic reticulum Ca2+ ATPase Cys-674 regulate endothelial cell calcium stores and angiogenic function as well as promote ischemic blood flow recovery
Authors: Thompson, Melissa D and Mei, Yu and Weisbrod, Robert M and Silver, Marcy and Shukla, Praphulla C and Bolotina, Victoria M and Cohen, Richard A and Tong, Xiaoyong
Journal: Journal of Biological Chemistry (2014): 19907--19916

References


View all 35 references: Citation Explorer
DNA damage caused by benzo(a)pyrene in MCF-7 cells is increased by verapamil, probenecid and PSC833
Authors: Myllynen P, Kurttila T, Vaskivuo L, Vahakangas K.
Journal: Toxicol Lett (2007): 3
Is the monkey an appropriate animal model to examine drug-drug interactions involving renal clearance? Effect of probenecid on the renal elimination of H2 receptor antagonists
Authors: Tahara H, Kusuhara H, Chida M, Fuse E, Sugiyama Y.
Journal: J Pharmacol Exp Ther (2006): 1187
Inhibition of oat3-mediated renal uptake as a mechanism for drug-drug interaction between fexofenadine and probenecid
Authors: Tahara H, Kusuhara H, Maeda K, Koepsell H, Fuse E, Sugiyama Y.
Journal: Drug Metab Dispos (2006): 743
MRP2 (ABCC2) transports taxanes and confers paclitaxel resistance and both processes are stimulated by probenecid
Authors: Huisman MT, Chhatta AA, van Tellingen O, Beijnen JH, Schinkel AH.
Journal: Int J Cancer (2005): 824
Mediation of annexin 1 secretion by a probenecid-sensitive ABC-transporter in rat inflamed mucosa
Authors: Wein S, Fauroux M, Laffitte J, de Nadai P, Guaini C, Pons F, Comera C.
Journal: Biochem Pharmacol (2004): 1195
Open randomized study of pyrimethamine-sulphadoxine vs. pyrimethamine-sulphadoxine plus probenecid for the treatment of uncomplicated Plasmodium falciparum malaria in children
Authors: Sowunmi A, Fehintola FA, Adedeji AA, Gbotosho GO, Falade CO, Tambo E, Fateye BA, Happi TC, Oduola AM.
Journal: Trop Med Int Health (2004): 606
Inhibition of multidrug transporters by verapamil or probenecid does not alter blood-brain barrier penetration of levetiracetam in rats
Authors: Potschka H, Baltes S, Loscher W.
Journal: Epilepsy Res (2004): 85
Probenecid interacts with the pharmacokinetics of morphine-6-glucuronide in humans
Authors: Skarke C, Langer M, Jarrar M, Schmidt H, Geisslinger G, Lotsch J.
Journal: Anesthesiology (2004): 1394
Impact of Mrp2 on the biliary excretion and intestinal absorption of furosemide, probenecid, and methotrexate using Eisai hyperbilirubinemic rats
Authors: Chen C, Scott D, Hanson E, Franco J, Berryman E, Volberg M, Liu X.
Journal: Pharm Res (2003): 31
The influence of inhibition of probenecid sensitive transporters on the central nervous system (CNS) uptake and the antinociceptive activity of morphine-6-glucuronide in rats
Authors: Lotsch J, Schmidt R, Vetter G, Schmidt H, Skarke C, Niederberger E, Geisslinger G, Tegeder I.
Journal: Neurosci Lett (2002): 145