ProLite™ 5-245 kD Protein Ladder

The ProLite™ 5-245 kD Protein Ladder is a high-quality three-color protein standard consisting of 13 prestained proteins. These proteins span a broad range of molecular weights, from 3.5 to 245 kDa. While most proteins in this ladder are covalently linked to a blue chromophore, it includes two distinctive reference bands – a green one at 25 kDa and a red one at 75 kDa that become evident when separated by SDS-PAGE. The ProLite™ 5-245 kD Protein Ladder is primarily used to track protein separation during SDS-polyacrylamide gel electrophoresis, confirm Western transfer efficiency across various membranes (like PVDF, nylon, or nitrocellulose), and to provide an estimation of protein sizes. It is conveniently supplied in a ready-to-use format premixed in a gel loading buffer. This protein ladder can be directly loaded onto gels without the need to heat, reduce, or introduce any reducing agents prior to its application.