Name: ProLite™ 5-245 kD Protein Ladder
Brand: AAT Bioquest
SKU: 18015
Price: 99 USD
Availability: InStock

ProLite™ 5-245 kD Protein Ladder

The ProLite™ 5-245 kD Protein Ladder is a high-quality three-color protein standard consisting of 13 prestained proteins. These proteins span a broad range of molecular weights, from 3.5 to 245 kDa. While most proteins in this ladder are covalently linked to a blue chromophore, it includes two distinctive reference bands – a green one at 25 kDa and a red one at 75 kDa that become evident when separated by SDS-PAGE. The ProLite™ 5-245 kD Protein Ladder is primarily used to track protein separation during SDS-polyacrylamide gel electrophoresis, confirm Western transfer efficiency across various membranes (like PVDF, nylon, or nitrocellulose), and to provide an estimation of protein sizes. It is conveniently supplied in a ready-to-use format premixed in a gel loading buffer. This protein ladder can be directly loaded onto gels without the need to heat, reduce, or introduce any reducing agents prior to its application.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
18015	100 uL	Price

References

View all 20 references: Citation Explorer

Characterization of antigens of Enterobius vermicularis (pinworm) eggs.

Authors:

De Kostha, Y B N Sanduni and Pathirana, Sisira L and Handunnetti, Shiroma M and Gunawardena, Sharmini

Journal:

Scientific reports (2022): 14414

Trypsin Inhibitor Isolated From Glycine max (Soya Bean) Extraction, Purification, and Characterization.

Authors:

Khan, Sabir and Arshad, Shafia and Arif, Amina and Tanveer, Rida and Amin, Zeemal Seemab and Abbas, Saba and Maqsood, Amna and Raza, Muhammad and Munir, Arooj and Latif, Amara and Habiba, Maryam and Afzal, Muhammad

Journal:

Dose-response : a publication of International Hormesis Society (2022): 15593258221131462

Mesowestern Blot: Simultaneous Analysis of Hundreds of Submicroliter Lysates.

Authors:

Zadeh, Cameron O and Huggins, Jonah R and Sarmah, Deepraj and Westbury, Baylee C and Interiano, William R and Jordan, Micah C and Phillips, S Ashley and Dodd, William B and Meredith, Wesley O and Harold, Nicholas J and Erdem, Cemal and Birtwistle, Marc R

Journal:

ACS omega (2022): 28912-28923

The Penn State Protein Ladder system for inexpensive protein molecular weight markers.

Authors:

Santilli, Ryan T and Williamson, John E and Shibata, Yoshitaka and Sowers, Rosalie P and Fleischman, Andrew N and Tan, Song

Journal:

Scientific reports (2021): 16703

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV).

Authors:

Dai, Yifan and Molazemhosseini, Alireza and Liu, Chung Chiun

Journal:

Biosensors (2017)

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 20, 2025