Name: ProLite™ His-Tag Protein Gel Staining Kit *Green Fluorescence*
Brand: AAT Bioquest
SKU: 18010
Price: 430 USD
Availability: InStock

ProLite™ His-Tag Protein Gel Staining Kit

Green Fluorescence

The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization. The ProLite™ His-Tag Protein Gel Staining Kit provides a fast, sensitive, and highly specific fluorescent stain for visualizing His-tagged fusion proteins directly in a polyacrylamide gel following electrophoresis. This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter. It detects nanogram of His-tagged proteins directly in gels, thus eliminating the additional western blotting step. AAT Bioquest offers the largest selection of the His-Tag detection and purification products.

Two-fold dilution series of His-tagged annexin V were separated on a NuPAGE® 4–12% Bis-Tris gel and stained with the ProLite™ His-Tag Protein Gel Staining Kit according to standard protocols. <strong>Lane 1:</strong> His-tagged protein ladder, <strong>Lane 2 to 5:</strong> two-fold dilution of His-tagged annexin V.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
18010	10 Gels	Price

References

View all 43 references: Citation Explorer

Simplified detection of polyhistidine-tagged proteins in gels and membranes using a UV-excitable dye and a multiple chelator head pair.

Authors:

Raducanu, Vlad-Stefan and Isaioglou, Ioannis and Raducanu, Daniela-Violeta and Merzaban, Jasmeen S and Hamdan, Samir M

Journal:

The Journal of biological chemistry (2020): 12214-12223

A bispecific circular aptamer tethering a built-in universal molecular tag for functional protein delivery.

Authors:

Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong

Journal:

Chemical science (2020): 9648-9654

The surface syndecan protein from Macrobrachium rosenbergii could function as mediator in bacterial infections.

Authors:

Yang, Hui and Xiong, Haoran and Mi, Kaihang and Zhang, Yingying and Zhang, Xiaojun and Chen, Guohong

Journal:

Fish & shellfish immunology (2020): 62-68

[Cloning and expression of SmDXS2 gene in Swertia mussotii].

Authors:

Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo

Journal:

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941

Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.

Authors:

Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien

Journal:

The protein journal (2018): 369-379

Physical properties

Solvent

DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Gel Imager
Excitation	Blue laser
Emission	Long path green filter (SYBR filter)

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Page updated on September 23, 2025