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Propidium iodide
10 mM aqueous solution
Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.
Chemical structure for Propidium iodide *10 mM aqueous solution*
Chemical structure for Propidium iodide *10 mM aqueous solution*
CatalogSize
Price
Quantity
175171 mL
Price
 
Physical properties

Molecular weight668.39
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
6000
1
Excitation (nm)537
Emission (nm)618
Quantum yield
0.2
1
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
CAS25535-16-4
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Page updated on September 16, 2024