Propidium Iodide | AAT Bioquest

Propidium iodide

10 mM aqueous solution

Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17517	1 mL	Price

Citations

View all 14 citations: Citation Explorer

Chlorite and bromate alter the conjugative transfer of antibiotic resistance genes: Co-regulation of oxidative stress and energy supply

Authors:

Cao, Jinrui and Xue, Bin and Yang, Shuran and Yang, Xiaobo and Zhang, Xi and Qiu, Zhigang and Shen, Zhiqiang and Wang, Jingfeng

Journal:

Journal of Hazardous Materials (2024): 134257

Cylindrospermopsin enhances the conjugative transfer of plasmid-mediated multi-antibiotic resistance genes through glutathione biosynthesis inhibition

Authors:

Yang, Shuran and Cao, Jinrui and Zhao, Chen and Zhang, Xi and Li, Chenyu and Wang, Shang and Yang, Xiaobo and Qiu, Zhigang and Li, Chao and Wang, Jingfeng and others,

Journal:

Ecotoxicology and Environmental Safety (2024): 116288

Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer

Authors:

Zhang, Lei and Chen, Wenbang and Li, Xiaojun and Wang, Gengming and Xing, Fubao and Zhu, Xiao

Journal:

Cell Adhesion \& Migration (2024): 1--11

Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation

Authors:

Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute

Development and characterization of biomimetic peptide-based bioink for dental tissue engineering

Authors:

Altan, Zeynep

References

View all 60 references: Citation Explorer

Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide

Authors:

Baskic D, Popovic S, Ristic P, Arsenijevic NN.

Journal:

Cell Biol Int (2006): 924

Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma

Authors:

Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.

Journal:

Neoplasma (2006): 213

Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry

Authors:

Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.

Journal:

Arch Androl (2005): 371

Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma

Authors:

Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.

Journal:

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271

Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction

Authors:

Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.

Journal:

Transfusion (2005): 228

Physical properties

Molecular weight	668.39
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	6000 ¹
Excitation (nm)	537
Emission (nm)	618
Quantum yield	0.2 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	25535-16-4

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on September 16, 2024