Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Propidium iodide *10 mM aqueous solution*

Chemical structure for Propidium iodide *10 mM aqueous solution*
Chemical structure for Propidium iodide *10 mM aqueous solution*
Ordering information
Price ()
Catalog Number17517
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight668.39
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
25535-16-4
Molecular weight
668.39
Extinction coefficient (cm -1 M -1)
60001
Excitation (nm)
537
Emission (nm)
618
Quantum yield
0.21
Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Propidium iodide *10 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM149.613 µL748.066 µL1.496 mL7.481 mL14.961 mL
5 mM29.923 µL149.613 µL299.226 µL1.496 mL2.992 mL
10 mM14.961 µL74.807 µL149.613 µL748.066 µL1.496 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)60001
Excitation (nm)537
Emission (nm)618
Quantum yield0.21

Citations


View all 6 citations: Citation Explorer
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium
Authors: Lin, Yung-Kai and Sharma, Ruchi and Ma, Hsu and Chen, Wen-Shyan and Yao, Chao-Ling
Journal: Journal of the Taiwan Institute of Chemical Engineers (2017)
Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death
Authors: Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique
Journal: Journal of Controlled Release (2017)
Microbial responses to membrane cleaning using sodium hypochlorite in membrane bioreactors: Cell integrity, key enzymes and intracellular reactive oxygen species
Authors: Han, Xiaomeng and Wang, Zhiwei and Wang, Xueye and Zheng, Xiang and Ma, Jinxing and Wu, Zhichao
Journal: Water research (2016): 293--300
Microfluidic shear stress-regulated surfactant secretion in alveolar epithelial type II cells in vitro
Authors: Mahto, Sanjeev Kumar and Tenenbaum-Katan, Janna and Greenblum, Ayala and Rothen-Rutishauser, Barbara and Sznitman, Josué
Journal: American Journal of Physiology-Lung Cellular and Molecular Physiology (2014): L672--L683

References


View all 60 references: Citation Explorer
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Assessment of the cell viability of cultured Perkinsus marinus (Perkinsea), a parasitic protozoan of the Eastern oyster, Crassostrea virginica, using SYBRgreen-propidium iodide double staining and flow cytometry
Authors: Soudant P, Chu FL, Lund ED.
Journal: J Eukaryot Microbiol (2005): 492
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
The assessment of viability in isolated rat hepatocytes subjected to cold or subzero non-freezing preservation protocols using a propidium iodide modified test
Authors: Giraudi PJ, Almada LL, Mamprin ME, Guibert EE, Furno G, Rodriguez JV.
Journal: Cryo Letters (2005): 169
Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements
Authors: Kral T, Widerak K, Langner M, Hof M.
Journal: J Fluoresc (2005): 179
Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228
Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability
Authors: Lehtinen J, Nuutila J, Lilius EM.
Journal: Cytometry A (2004): 165
Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry
Authors: Lin SJ, Cheng PJ, Huang YJ, Kuo ML.
Journal: Pediatr Allergy Immunol (2004): 79