Propidium iodide *10 mM aqueous solution*
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 668.39 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 60001 |
| Excitation (nm) | 537 |
| Emission (nm) | 618 |
| Quantum yield | 0.21 |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | T |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41116134 |
Alternative formats
| Propidium iodide *CAS 25535-16-4* |
| Propidium iodide *1 mg/mL aqueous solution* |
| Overview |  SDSProtocol |
See also: Annexin-V Staining, Cell Cycle Assays, Nucleus, Cell Viability Assays, Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry (IHC)
CAS 25535-16-4 | Molecular weight 668.39 | Extinction coefficient (cm -1 M -1) 60001 | Excitation (nm) 537 | Emission (nm) 618 | Quantum yield 0.21 |
Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Propidium iodide *10 mM aqueous solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 149.613 µL | 748.066 µL | 1.496 mL | 7.481 mL | 14.961 mL |
| 5 mM | 29.923 µL | 149.613 µL | 299.226 µL | 1.496 mL | 2.992 mL |
| 10 mM | 14.961 µL | 74.807 µL | 149.613 µL | 748.066 µL | 1.496 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 60001 |
| Excitation (nm) | 537 |
| Emission (nm) | 618 |
| Quantum yield | 0.21 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] | 754 | 778 | 2700001 |
| DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] | 550 | 564 | 148000 |
Images
Figure 1. Chemical structure for Propidium iodide *10 mM aqueous solution*
Citations
View all 11 citations: Citation Explorer
Development and characterization of biomimetic peptide-based bioink for dental tissue engineering
Authors: Altan, Zeynep
Journal: (2023)
Authors: Altan, Zeynep
Journal: (2023)
Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685
The role of non-coding RNA ASBEL regulation of BTG3 in pancreatic ductal adenocarcinoma tumor progression
Authors: Chen, Jing and Zhu, Ming-Yuan and Huang, Yan-Hua and Ling, Yi-Ting and Gu, Tian-Yuan and Zhou, Quan and Fei, Ming-Jian and Zhou, Zhong-Cheng
Journal: Current Therapeutic Research (2023): 100700
Authors: Chen, Jing and Zhu, Ming-Yuan and Huang, Yan-Hua and Ling, Yi-Ting and Gu, Tian-Yuan and Zhou, Quan and Fei, Ming-Jian and Zhou, Zhong-Cheng
Journal: Current Therapeutic Research (2023): 100700
Protective role of melatonin against testicular damage caused by polymicrobial sepsis in adult rats
Authors: Doganay, Songul and Budak, Ozcan and Toprak, Veysel and Erman, Gulay and Sahin, Arzu
Journal: Ulus Travma Acil Cerrahi Derg (2022): 723--729
Authors: Doganay, Songul and Budak, Ozcan and Toprak, Veysel and Erman, Gulay and Sahin, Arzu
Journal: Ulus Travma Acil Cerrahi Derg (2022): 723--729
Black carbon and humic acid alleviate the toxicity of antibiotics to a cyanobacterium Synechocystis sp.
Authors: You, Mingtao and You, Xiuqi and Yang, Xi and Hu, Jingrun and Lyu, Yitao and Sun, Weiling
Journal: Environmental Pollution (2022): 120646
Authors: You, Mingtao and You, Xiuqi and Yang, Xi and Hu, Jingrun and Lyu, Yitao and Sun, Weiling
Journal: Environmental Pollution (2022): 120646
Development and Characterization of Electrospun Polymer Scaffolds for 3D Cell Culture Model to Mimic Human Intestine System
Authors: Firik, Tu{\u{g}}ba
Journal: (2020)
Authors: Firik, Tu{\u{g}}ba
Journal: (2020)
Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
Authors: Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: Biochemical and Biophysical Research Communications (2017)
In situ polymerizable hydrogel incorporated with specific pathogen-free porcine platelet-rich plasma for the reconstruction of the corneal endothelium
Authors: Lin, Yung-Kai and Sharma, Ruchi and Ma, Hsu and Chen, Wen-Shyan and Yao, Chao-Ling
Journal: Journal of the Taiwan Institute of Chemical Engineers (2017)
Authors: Lin, Yung-Kai and Sharma, Ruchi and Ma, Hsu and Chen, Wen-Shyan and Yao, Chao-Ling
Journal: Journal of the Taiwan Institute of Chemical Engineers (2017)
Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death
Authors: Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique
Journal: Journal of Controlled Release (2017)
Authors: Clerc, Pascal and Jeanjean, Pauline and Halalli, Nicolas and Gougeon, Michel and Pipy, Bernard and Carrey, Julian and Fourmy, Daniel and Gigoux, Véronique
Journal: Journal of Controlled Release (2017)
Microbial responses to membrane cleaning using sodium hypochlorite in membrane bioreactors: Cell integrity, key enzymes and intracellular reactive oxygen species
Authors: Han, Xiaomeng and Wang, Zhiwei and Wang, Xueye and Zheng, Xiang and Ma, Jinxing and Wu, Zhichao
Journal: Water research (2016): 293--300
Authors: Han, Xiaomeng and Wang, Zhiwei and Wang, Xueye and Zheng, Xiang and Ma, Jinxing and Wu, Zhichao
Journal: Water research (2016): 293--300
References
View all 60 references: Citation Explorer
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Assessment of the cell viability of cultured Perkinsus marinus (Perkinsea), a parasitic protozoan of the Eastern oyster, Crassostrea virginica, using SYBRgreen-propidium iodide double staining and flow cytometry
Authors: Soudant P, Chu FL, Lund ED.
Journal: J Eukaryot Microbiol (2005): 492
Authors: Soudant P, Chu FL, Lund ED.
Journal: J Eukaryot Microbiol (2005): 492
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
The assessment of viability in isolated rat hepatocytes subjected to cold or subzero non-freezing preservation protocols using a propidium iodide modified test
Authors: Giraudi PJ, Almada LL, Mamprin ME, Guibert EE, Furno G, Rodriguez JV.
Journal: Cryo Letters (2005): 169
Authors: Giraudi PJ, Almada LL, Mamprin ME, Guibert EE, Furno G, Rodriguez JV.
Journal: Cryo Letters (2005): 169
Propidium iodide and PicoGreen as dyes for the DNA fluorescence correlation spectroscopy measurements
Authors: Kral T, Widerak K, Langner M, Hof M.
Journal: J Fluoresc (2005): 179
Authors: Kral T, Widerak K, Langner M, Hof M.
Journal: J Fluoresc (2005): 179
Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228
Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability
Authors: Lehtinen J, Nuutila J, Lilius EM.
Journal: Cytometry A (2004): 165
Authors: Lehtinen J, Nuutila J, Lilius EM.
Journal: Cytometry A (2004): 165
Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry
Authors: Lin SJ, Cheng PJ, Huang YJ, Kuo ML.
Journal: Pediatr Allergy Immunol (2004): 79
Authors: Lin SJ, Cheng PJ, Huang YJ, Kuo ML.
Journal: Pediatr Allergy Immunol (2004): 79