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Propidium Iodide
10 mM aqueous solution
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Propidium iodide (PI) belongs to the same chemical class of ethidium bromide. As in the case of ethidium bromide its fluorescence is enhanced for 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted for 30-40 nm and the fluorescence emission maximum blue-shifted for 15 nm or so. PI also binds to RNA as DAPI and acridine orange. PI is commonly used for identifying dead cells in a population of cells and as a counterstain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and normal cells. It is suitable for fluorescence microscopy, flow cytometry and fluorometry.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] | 754 | 778 | 2700001 |
| DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] | 550 | 564 | 148000 |
Citations
View all 14 citations: Citation Explorer
Chlorite and bromate alter the conjugative transfer of antibiotic resistance genes: Co-regulation of oxidative stress and energy supply
Authors: Cao, Jinrui and Xue, Bin and Yang, Shuran and Yang, Xiaobo and Zhang, Xi and Qiu, Zhigang and Shen, Zhiqiang and Wang, Jingfeng
Journal: Journal of Hazardous Materials (2024): 134257
Authors: Cao, Jinrui and Xue, Bin and Yang, Shuran and Yang, Xiaobo and Zhang, Xi and Qiu, Zhigang and Shen, Zhiqiang and Wang, Jingfeng
Journal: Journal of Hazardous Materials (2024): 134257
Cylindrospermopsin enhances the conjugative transfer of plasmid-mediated multi-antibiotic resistance genes through glutathione biosynthesis inhibition
Authors: Yang, Shuran and Cao, Jinrui and Zhao, Chen and Zhang, Xi and Li, Chenyu and Wang, Shang and Yang, Xiaobo and Qiu, Zhigang and Li, Chao and Wang, Jingfeng and others,
Journal: Ecotoxicology and Environmental Safety (2024): 116288
Authors: Yang, Shuran and Cao, Jinrui and Zhao, Chen and Zhang, Xi and Li, Chenyu and Wang, Shang and Yang, Xiaobo and Qiu, Zhigang and Li, Chao and Wang, Jingfeng and others,
Journal: Ecotoxicology and Environmental Safety (2024): 116288
Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
Authors: Zhang, Lei and Chen, Wenbang and Li, Xiaojun and Wang, Gengming and Xing, Fubao and Zhu, Xiao
Journal: Cell Adhesion \& Migration (2024): 1--11
Authors: Zhang, Lei and Chen, Wenbang and Li, Xiaojun and Wang, Gengming and Xing, Fubao and Zhu, Xiao
Journal: Cell Adhesion \& Migration (2024): 1--11
Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685
Development and characterization of biomimetic peptide-based bioink for dental tissue engineering
Authors: Altan, Zeynep
Journal: (2023)
Authors: Altan, Zeynep
Journal: (2023)
References
View all 60 references: Citation Explorer
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Prognostic significance of plasma cell propidium iodide and annexin-V indices and their mutual ratio in multiple myeloma
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Authors: Scudla V, Ordeltova M, Minarik J, Dusek L, Zemanova M, Bacovsky J.
Journal: Neoplasma (2006): 213
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
Evaluation of plasma cell propidium-iodide and annexin-V indices: Their relation to prognosis in multiple myeloma
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Authors: Minarik J, Scudla V, Ordeltova M, Bacovsky J, Zemanova M.
Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub (2005): 271
Low propidium iodide intensity in flow cytometric white blood cell counting as a marker of cell destruction
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228
Authors: Wagner T, Guber SE, Stubenrauch ML, Lanzer G, Neumueller J.
Journal: Transfusion (2005): 228