Protonex™ Lyso-NIR 770

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
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Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	736.76
Solvent	DMSO

Spectral properties

Excitation (nm)	748
Emission (nm)	769

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

736.76

Excitation (nm)

748

Emission (nm)

769

Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester. It is cell-permeable and selectively localized in acidic organelles such as lysosomes. It is the only cell-permeable acidotropic pH dye with NIR fluorescence for monitoring pH changes in acidic organelles. Its fluorescence can be readily captured with the common Cy7 filter set that is equipped with most of the fluorescence instruments. Due to its significantly red-shifted excitation and emission wavelengths, it has much lower background than the existing pH sensors (such as Lysosensors). It might be used in vivo for monitoring pH changes in animals due to the significantly improved skin-penetration capability of NIR excitation.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Lyso-NIR 770 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	135.729 µL	678.647 µL	1.357 mL	6.786 mL	13.573 mL
5 mM	27.146 µL	135.729 µL	271.459 µL	1.357 mL	2.715 mL
10 mM	13.573 µL	67.865 µL	135.729 µL	678.647 µL	1.357 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	748
Emission (nm)	769

Images

Figure 1. Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.

References

View all 50 references: Citation Explorer

Rational design of a ratiometric fluorescent probe for imaging lysosomal nitroreductase activity.
Authors: Bai, Wenjun and Li, Yixuan and Zhao, Li and Li, Ruxin and Geng, Jiahou and Lu, Yang and Zhao, Yufen and Wang, Jinhui
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 123032

A potent fluorescent transmembrane HCl transporter perturbs cellular pH and promotes cancer cell death.
Authors: Fares, Mohamed and Wu, Xin and McNaughton, Daniel A and Gilchrist, Alexander M and Lewis, William and Keller, Paul A and Arias-Betancur, Alain and Fontova, Pere and Pérez-Tomás, Ricardo and Gale, Philip A
Journal: Organic & biomolecular chemistry (2023): 2509-2515

Analysis of Radachlorin localization in living cells by fluorescence lifetime imaging microscopy.
Authors: Belashov, A V and Zhikhoreva, A A and Salova, A V and Belyaeva, T N and Litvinov, I K and Kornilova, E S and Semenova, I V and Vasyutinskii, O S
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112699

In vivo Assessment of Lysosomal Stress in the Drosophila Brain Using Confocal Fluorescence Microscopy.
Authors: Martelli, Felipe
Journal: Bio-protocol (2023): e4599

A water-soluble fluorescent pH probe and its application for monitoring lysosomal pH changes in living cells.
Authors: Zhang, Lei and Guo, Jun and You, Qihua and Xu, Yuqing
Journal: Analytical methods : advancing methods and applications (2023): 3057-3063

A novel AIE fluorescence probe featuring with high quantum yield for high-fidelity lysosomal targeting and tracking.
Authors: Zhang, Pan and Xue, Ke and Dai, Yanpeng and Zhao, Xinxin and Zhang, Dongdong and Wei, Pengfei and Qi, Zhengjian
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122657

A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.
Authors: Dolman, Nick J and Kilgore, Jason A
Journal: Current protocols (2023): e751

A series of meso amide BODIPY based lysosome-targeting fluorescent probe with high photostability and sensitivity.
Authors: Ruan, Li and Bai, Junping and Ji, Xin and Zhao, Weili and Dong, Xiaochun
Journal: Analytica chimica acta (2022): 339771

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome.
Authors: Liu, Bilin and Li, Anqi and Qin, Yuan and Chen, Lei and Gao, Meng and Gong, Guohua
Journal: Journal of visualized experiments : JoVE (2022)

Cell-imaging studies of highly substituted oxazole derivatives as organelle targeting fluorophores (OTFPs).
Authors: Adhikary, Saswati and Mukherjee, Kaustuv and Banerji, Biswadip
Journal: Scientific reports (2022): 16555