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Protonex™ Lyso-NIR 770

Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester. It is cell-permeable and selectively localized in acidic organelles such as lysosomes. It is the only cell-permeable acidotropic pH dye with NIR fluorescence for monitoring pH changes in acidic organelles. Its fluorescence can be readily captured with the common Cy7 filter set that is equipped with most of the fluorescence instruments. Due to its significantly red-shifted excitation and emission wavelengths, it has much lower background than the existing pH sensors (such as Lysosensors). It might be used in vivo for monitoring pH changes in animals due to the significantly improved skin-penetration capability of NIR excitation.

Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.
Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
212421 mg
Price
 
Physical properties
Molecular weight736.76
SolventDMSO
Spectral properties
Excitation (nm)748
Emission (nm)769
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 30, 2025