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Protonex™ Lyso-Red 670
Lysosomes are membrane-bound organelles containing acid hydrolases that break down biomolecules in an acidic environment (pH 4.5-4.8). They play critical roles in cellular processes by eliminating waste and debris, facilitating autophagy for organelle recycling, and mediating digestion through endocytosis and phagocytosis. Lysosomes aid cellular metabolism by converting complex molecules into usable forms and contribute to cell signaling, influencing growth, differentiation, and responses to signals. Dysfunctional lysosomes are associated with various diseases called lysosomal storage disorders, where the accumulation of undigested substances within lysosomes leads to cellular damage and systemic health issues. Notably, lysosomal dysfunction has been implicated in neurodegenerative diseases, cancer, and other disorders. Protonex™ Lyso-Red 670 is a cell-permeable, far-red fluorescent probe that selectively stains and visualizes acidic organelles within live cells, notably lysosomes. It remains quiescent or weakly fluorescent under alkaline pH conditions, exhibiting significant fluorescence enhancement as acidity increases. This property enables researchers to trace lysosomal dynamics (e.g., trafficking, fusion, fission, autophagy) in real-time via fluorescence microscopy techniques. Protonex™ Lyso-Red 670 has an excitation and emission maximum of 640 and 680 nm, respectively, and can be efficiently excited using a Cy5 filter.
Response of HeLa cells labeled with Protonex™ Lyso-Red 670. HeLa cells were incubated with 5 µM of Protonex™ Lyso-Red 670 for 30 minutes at 37°C. Incubation of Protonex™ Lyso-Red 670 solution with HeLa cells showed a homogenous uptake of Protonex™ Lyso-Red 670 and stained cell cytosol. The Spexyte™ Intracellular pH Calibration Buffer Kit (Cat No. 21235) was used to clamp the intracellular pH with extracellular buffers at pH 4 to 10. Images were acquired using a fluorescence microscope with a Cy5 filter set (A), and fluorescence was measured using a fluorescence microplate reader (FlexStation 3) at Ex/Em = 640/680 nm, cutoff = 665 nm (B).
Response of HeLa cells labeled with Protonex™ Lyso-Red 670. HeLa cells were incubated with 5 µM of Protonex™ Lyso-Red 670 for 30 minutes at 37°C. Incubation of Protonex™ Lyso-Red 670 solution with HeLa cells showed a homogenous uptake of Protonex™ Lyso-Red 670 and stained cell cytosol. The Spexyte™ Intracellular pH Calibration Buffer Kit (Cat No. 21235) was used to clamp the intracellular pH with extracellular buffers at pH 4 to 10. Images were acquired using a fluorescence microscope with a Cy5 filter set (A), and fluorescence was measured using a fluorescence microplate reader (FlexStation 3) at Ex/Em = 640/680 nm, cutoff = 665 nm (B).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
212411 mg
Price
 
Physical properties
Molecular weight710.72
SolventDMSO
Spectral properties
Excitation (nm)643
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff665 nm
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 31, 2025