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Protonex™ NIR 770 NHS ester

Protonex™ NIR 770 NHS ester is the only cell-permeable acidotropic pH dye commercially available for developing NIR pH probes. It is an excellent building block that can be readily used for developing site-specific pH sensors for monitoring pH fluctuation in a spatial environment. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). It easily penetrates live cell membranes, thus can be used for monitoring pH changes in live cells. It is complimentary to CypHer7E that is cell-impermeable, and often used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. Protonex™ NIR 770 NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. It is one of the very few acidotropic pH probes that exhibit NIR fluorescence.

Image of Hela cells stained with Protonex™ NIR 770 NHS ester. Hela cells were washed 3 times with HH buffer and then incubated with 10 µM of Protonex™ NIR 770 NHS ester in HH buffer for 30 min at 37°C. The cells were then washed 3 times with HH buffer and incubated with HH buffer or pH=5.0 and pH=8.0 buffer (Cat# 21235) containing 10 µM of nigericin for 5 min at 37°C. Images were acquired using a fluorescence microscope equipped with a Cy7 filter set.
Image of Hela cells stained with Protonex™ NIR 770 NHS ester. Hela cells were washed 3 times with HH buffer and then incubated with 10 µM of Protonex™ NIR 770 NHS ester in HH buffer for 30 min at 37°C. The cells were then washed 3 times with HH buffer and incubated with HH buffer or pH=5.0 and pH=8.0 buffer (Cat# 21235) containing 10 µM of nigericin for 5 min at 37°C. Images were acquired using a fluorescence microscope equipped with a Cy7 filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
212211 mg
Price
 
Physical properties
Molecular weight535.64
SolventDMSO
Spectral properties
Excitation (nm)748
Emission (nm)769
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 20, 2025