Name: Protonex™ Red 600, SE
Brand: AAT Bioquest
SKU: 21208
Price: 348 USD
Availability: InStock

Protonex™ Red 600, SE

Protonex™ Red dye demonstrated pH-dependent fluorescence. Unlike most of the existing fluorescent dyes that are more fluorescent at higher pH, acidic conditions enhance the fluorescence of Protonex™ Red dye. The fluorescence of Protonex™ Red dye dramatically increases as pH decreases from neutral to the acidic. The weak fluorescence outside the cell may potentially eliminates the wash steps. Protonex™ Red dye provides a powerful tool to monitor acidic cell compartments such as endosomes and lysosomes. Protonex™ Red dye is weakly fluorescent outside the cells, but its fluorescence is significantly enhanced in acidic compartments (such as phagosomes, lysosomes and endosomes). This Protonex™ Red enables the specific detection of cellular acidic compartments with reduced signal variability and improved accuracy for imaging or flow applications. It can be also used for multiplexing cellular functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Protonex™ Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of Protonex™ Red. This Protonex™ Red SE can be readily used to make a variety of bioconjugates for imaging or flow applications, enabling the specific detection of phagocytosis and endocytosis with reduced signal variability and improved accuracy. These conjugates can be also used for multiplexing cell functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. Protonex™ Red has the spectral properties similar to those of Texas Red, making the common filter set of Texas Red readily available to the assays of Protonex™ Red.

The pH dependent Emission spectra of Protonex™ Red 600.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21208	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 600, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.925 µL	524.626 µL	1.049 mL	5.246 mL	10.493 mL
5 mM	20.985 µL	104.925 µL	209.85 µL	1.049 mL	2.099 mL
10 mM	10.493 µL	52.463 µL	104.925 µL	524.626 µL	1.049 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	953.06 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Real-Time Visualization of Drug-Target Interactions in Native Subcellular Microenvironments for Lysosome-targeted Drug Discovery

Authors:

Wang, Ran and Yuan, Yatong and Shao, Huarong and Sun, Yuehao and Lai, Changcheng and Zhang, Mengrui and Song, Wenjing and Zhang, Tao and Zhuang, Fengfeng and Chen, Qixin and others,

Journal:

Journal of Pharmaceutical Analysis (2025): 101428

A novel anti-CD146 antibody specifically targets cancer cells by internalizing the molecule

Authors:

Nollet, Marie and Stalin, Jimmy and Moyon, Anais and Traboulsi, Wael and Essaadi, Amel and Robert, Stéphane and Malissen, Nausicaa and Bachelier, Richard and Daniel, Laurent and Foucault-Bertaud, Alex and rine , undefined and others, undefined

Journal:

Oncotarget (2017): 112283

PHD2 is a regulator for glycolytic reprogramming in macrophages

Authors:

Guentsch, Annemarie and Beneke, Angelika and Swain, Lija and Farhat, Katja and Nagarajan, Shunmugam and Wielockx, Ben and Raithatha, Kaamini and Dudek, Jan and Rehling, Peter and Zieseniss, Anke and others, undefined

Journal:

Molecular and Cellular Biology (2016): MCB--00236

References

View all 56 references: Citation Explorer

Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes

Authors:

Sarantis H, Grinstein S.

Journal:

Methods Cell Biol (2012): 429

Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock

Authors:

Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.

Journal:

J Cell Commun Signal (2011): 135

The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis

Authors:

Flannagan RS, Grinstein S.

Journal:

Methods Mol Biol (2010): 121

Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms

Authors:

Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.

Journal:

Inhal Toxicol (2010): 1091

Analysis of macrophage phagocytosis: quantitative assays of phagosome formation and maturation using high-throughput fluorescence microscopy

Authors:

Steinberg BE, Grinstein S.

Journal:

Methods Mol Biol (2009): 45

Physical properties

Molecular weight	953.06
Solvent	DMSO

Spectral properties

Excitation (nm)	576
Emission (nm)	597

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025