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Protonex™ Red 670 AM
Cell-Permeable
Protonex™ Red 670 AM is the cell-permeable version of Protonex™ Red 670. Protonex™ Red 670 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to an acidic intracellular target, it becomes highly fluorescent and emits red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can label or monitor the acidic intracellular targets in live cells.
Intracellular pH response of HeLa cells labeled with Protonex™ Red 670 AM. Following staining, intracellular pH was clamped using the Spexyte™ Intracellular pH Calibration Buffer Kit (Cat. No. 21235) with extracellular buffers ranging from pH 4 to 10. Fluorescence was measured using a CLARIOstar fluorescence microplate reader as the 570/470 nm excitation ratio, with emission collected at 700 nm using a 665 nm cutoff for both excitation wavelengths.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
211821 mg
Price
 
Physical properties
Molecular weight521.05
SolventDMSO
Spectral properties
Excitation (nm)643
Emission (nm)660
Usage and storage
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings
Fluorescence microscope
ExcitationCy5 Filter Set
EmissionCy5 Filter Set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff665 nm
Recommended plateBlack wall/clear bottom
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Page updated on August 17, 2026