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Protonex™ Red 670 AM
Cell-Permeable
Protonex™ Red 670 AM is the cell-permeable version of Protonex™ Red 670. Protonex™ Red 670 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to an acidic intracellular target, it becomes highly fluorescent and emits red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can label or monitor the acidic intracellular targets in live cells.
Response of HeLa cells labeled with Protonex™ Red 670 AM. HeLa cells were incubated with 5 µM of Protonex™ Red 670 AM for 30 minutes at 37°C. Incubation of Protonex™ Red 670 AM solution with HeLa cells showed a homogenous uptake of Protonex™ Red 670 AM and stained cell cytosol. The Spexyte™ Intracellular pH Calibration Buffer Kit (Cat No. 21235) was used to clamp the intracellular pH with extracellular buffers at pH 4 to 10. (A) Images were acquired using a fluorescence microscope with a Cy5 filter set, and (B) fluorescence was measured using a ClarioStar fluorescence microplate reader (Ex/Em = 640/680 nm, cutoff = 665 nm).
Response of HeLa cells labeled with Protonex™ Red 670 AM. HeLa cells were incubated with 5 µM of Protonex™ Red 670 AM for 30 minutes at 37°C. Incubation of Protonex™ Red 670 AM solution with HeLa cells showed a homogenous uptake of Protonex™ Red 670 AM and stained cell cytosol. The Spexyte™ Intracellular pH Calibration Buffer Kit (Cat No. 21235) was used to clamp the intracellular pH with extracellular buffers at pH 4 to 10. (A) Images were acquired using a fluorescence microscope with a Cy5 filter set, and (B) fluorescence was measured using a ClarioStar fluorescence microplate reader (Ex/Em = 640/680 nm, cutoff = 665 nm).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
211821 mg
Price
 
Physical properties
Molecular weight521.05
SolventDMSO
Spectral properties
Excitation (nm)643
Emission (nm)660
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationCy5 Filter Set
EmissionCy5 Filter Set
Recommended plateBlack wall/clear bottom
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff665 nm
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on November 20, 2025