Psoralen MOP Azide

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Telephone	1-800-990-8053
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Physical properties

Molecular weight	356.34
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

See also: Click Chemistry

Molecular weight

356.34

Psoralen MOP Azide is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Alkyne–Azide Cycloaddition (CuAAC). It can be readily conjugated to azido-containing biomolecules (such as alkynyl-modified antibodies, peptide alkynes and alkynyl-modified oligos). Psoralens and their derivatives (such as 8-MOP and 4,5’8-TMP) are well known to have unique crosslinking features to DNA. However, psoralen monomers do not have sequence-specific crosslinking ability with a target DNA. Nakao et al found that 5-MOP more effectively crosslinks DNA compared to the well-known 8-MOP (https://doi.org/10.1002/cbic.202200789). Psoralen MOP Azide is an alkynyl-reactive 5-MOP derivative. It is an excellent building block for preparing MOP-labeled oligos from an alkynyl-modified oligo. The 5-MOP-conjugated oligonucleotides can be used for sequence-specific crosslinking with a target DNA, thus enabling the application of psoralen-conjugated molecules in gene transcription inhibition, gene knockout, and other genomic applications.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Psoralen MOP Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	280.631 µL	1.403 mL	2.806 mL	14.032 mL	28.063 mL
5 mM	56.126 µL	280.631 µL	561.262 µL	2.806 mL	5.613 mL
10 mM	28.063 µL	140.315 µL	280.631 µL	1.403 mL	2.806 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Images

Figure 1. Psoralen MOP Azide is an alkynyl-reactive 5-MOP derivative. It is an excellent building block for preparing MOP-labeled oligos from an alkynyl-modified oligo.