Calculator
Psoralen TMP Alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 408.45 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Psoralen TMP Succinimidyl Ester |
| Psoralen TMP Maleimide |
| Psoralen TMP Azide |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 408.45 |
Psoralen TMP Alkyne is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Azide–Alkyne Cycloaddition (CuAAC). It can be readily conjugated to azido-containing biomolecules (such as azido-modified antibodies, peptide azides and azido-modified oligos). Psoralens and their derivatives (such as 8-TMP and 4,5’,8-TMP) are well known to have unique crosslinking features to DNA. Psoralen TMP Alkyne is an azide-reactive TMP derivative. It is an excellent building block for preparing TMP-labeled oligos from an azido-modified oligo. The TMP-conjugated oligonucleotides can be used for sequence-specific crosslinking with a target DNA, thus enabling the application of psoralen-conjugated molecules in gene transcription inhibition, gene knockout, and other genomic applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Psoralen TMP Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 244.828 µL | 1.224 mL | 2.448 mL | 12.241 mL | 24.483 mL |
| 5 mM | 48.966 µL | 244.828 µL | 489.656 µL | 2.448 mL | 4.897 mL |
| 10 mM | 24.483 µL | 122.414 µL | 244.828 µL | 1.224 mL | 2.448 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Images
Figure 1. Psoralen TMP Alkyne is an azide-reactive TMP derivative. It is an excellent building block for preparing TMP-labeled oligos from an azido-modified oligo.
References
View all 7 references: Citation Explorer
Computer modeling of three-dimensional structure of DNA-packaging RNA (pRNA) monomer, dimer, and hexamer of Phi29 DNA packaging motor.
Authors: Hoeprich, Stephen and Guo, Peixuan
Journal: The Journal of biological chemistry (2002): 20794-803
Authors: Hoeprich, Stephen and Guo, Peixuan
Journal: The Journal of biological chemistry (2002): 20794-803
Photoaffinity behavior of a conjugate of oligonucleoside methylphosphonate, rhodamine, and psoralen in the presence of complementary oligonucleotides.
Authors: Thaden, J and Miller, P S
Journal: Bioconjugate chemistry (1993): 386-94
Authors: Thaden, J and Miller, P S
Journal: Bioconjugate chemistry (1993): 386-94
Laser-induced protein-DNA cross-links via psoralen furanside monoadducts.
Authors: Sastry, S S and Spielmann, H P and Hoang, Q S and Phillips, A M and Sancar, A and Hearst, J E
Journal: Biochemistry (1993): 5526-38
Authors: Sastry, S S and Spielmann, H P and Hoang, Q S and Phillips, A M and Sancar, A and Hearst, J E
Journal: Biochemistry (1993): 5526-38
Lethal and mutagenic effects photoinduced in haploid yeast (Saccharomyces cerevisiae) by two new monofunctional pyridopsoralens compared to 3-carbethoxypsoralen and 8-methoxypsoralen.
Authors: Averbeck, D and Averbeck, S and Bisagni, E and Moron, L
Journal: Mutation research (1985): 47-57
Authors: Averbeck, D and Averbeck, S and Bisagni, E and Moron, L
Journal: Mutation research (1985): 47-57
Organization of herpes simplex virus type 1 deoxyribonucleic acid during replication probed in living cells with 4,5',8-trimethylpsoralen.
Authors: Sinden, R R and Pettijohn, D E and Francke, B
Journal: Biochemistry (1982): 4484-90
Authors: Sinden, R R and Pettijohn, D E and Francke, B
Journal: Biochemistry (1982): 4484-90
New procedure using a psoralen derivative for analysis of nucleosome associated DNA sequences in chromatin of living cells.
Authors: Carlson, J O and Pfenninger, O and Sinden, R R and Lehman, J M and Pettijohn, D E
Journal: Nucleic acids research (1982): 2043-63
Authors: Carlson, J O and Pfenninger, O and Sinden, R R and Lehman, J M and Pettijohn, D E
Journal: Nucleic acids research (1982): 2043-63
Biological effects and repair of damage photoinduced by a derivative of psoralen substituted at the 3,4 reaction site: photoreactivity of this compound and lethal effect in yeast.
Authors: Averbeck, D and Moustacchi, E and Bisagni, E
Journal: Biochimica et biophysica acta (1978): 464-81
Authors: Averbeck, D and Moustacchi, E and Bisagni, E
Journal: Biochimica et biophysica acta (1978): 464-81