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Psoralen TMP Azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 439.47 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| Psoralen TMP Succinimidyl Ester |
| Psoralen TMP Maleimide |
| Psoralen TMP Alkyne |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 439.47 |
Psoralen TMP Azide is an excellent building block for developing site-specific nucleic acid probes via the well-known click chemistry, i.e., Copper-Catalyzed Alkyne–Azide Cycloaddition (CuAAC). It can be readily conjugated to azido-containing biomolecules (such as alkynyl-modified antibodies, peptide alkynes and alkynyl-modified oligos). Psoralens and their derivatives (such as 8-TMP and 4,5’,8-TMP) are well known to have unique crosslinking features to DNA. Psoralen TMP Azide is an alkynyl-reactive TMP derivative. It is an excellent building block for preparing TMP-labeled oligos from an alkynyl-modified oligo. The TMP-conjugated oligonucleotides can be used for sequence-specific crosslinking with a target DNA, thus enabling the application of psoralen-conjugated molecules in gene transcription inhibition, gene knockout, and other genomic applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Psoralen TMP Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 227.547 µL | 1.138 mL | 2.275 mL | 11.377 mL | 22.755 mL |
| 5 mM | 45.509 µL | 227.547 µL | 455.094 µL | 2.275 mL | 4.551 mL |
| 10 mM | 22.755 µL | 113.773 µL | 227.547 µL | 1.138 mL | 2.275 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Psoralen TMP Azide is an alkynyl-reactive TMP derivative. It is an excellent building block for preparing TMP-labeled oligos from an alkynyl-modified oligo.
References
View all 50 references: Citation Explorer
Allosteric regulation of U1 snRNP by splicing regulatory proteins controls spliceosomal assembly.
Authors: Shenasa, Hossein and Movassat, Maliheh and Forouzmand, Elmira and Hertel, Klemens J
Journal: RNA (New York, N.Y.) (2020): 1389-1399
Authors: Shenasa, Hossein and Movassat, Maliheh and Forouzmand, Elmira and Hertel, Klemens J
Journal: RNA (New York, N.Y.) (2020): 1389-1399
In yeast cells arrested at the early S-phase by hydroxyurea, rRNA gene promoters and chromatin are poised for transcription while rRNA synthesis is compromised.
Authors: Charton, Romain and Muguet, Alexia and Griesenbeck, Joachim and Smerdon, Michael J and Conconi, Antonio
Journal: Mutation research (2019): 20-29
Authors: Charton, Romain and Muguet, Alexia and Griesenbeck, Joachim and Smerdon, Michael J and Conconi, Antonio
Journal: Mutation research (2019): 20-29
Analyses of rRNA gene chromatin in cell cycle arrested Saccharomyces cerevisiae cells.
Authors: Paillé, Audrey and Charton, Romain and Muguet, Alexia and Griesenbeck, Joachim and Smerdon, Michael J and Conconi, Antonio
Journal: Data in brief (2019): 104083
Authors: Paillé, Audrey and Charton, Romain and Muguet, Alexia and Griesenbeck, Joachim and Smerdon, Michael J and Conconi, Antonio
Journal: Data in brief (2019): 104083
Dynamic Architecture of Eukaryotic DNA Replication Forks In Vivo, Visualized by Electron Microscopy.
Authors: Zellweger, Ralph and Lopes, Massimo
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 261-294
Authors: Zellweger, Ralph and Lopes, Massimo
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 261-294
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen.
Authors: Aw, Jong Ghut Ashley and Shen, Yang and Nagarajan, Niranjan and Wan, Yue
Journal: Journal of visualized experiments : JoVE (2017)
Authors: Aw, Jong Ghut Ashley and Shen, Yang and Nagarajan, Niranjan and Wan, Yue
Journal: Journal of visualized experiments : JoVE (2017)
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks.
Authors: Mukherjee, Anirban and Vasquez, Karen M
Journal: Journal of visualized experiments : JoVE (2016)
Authors: Mukherjee, Anirban and Vasquez, Karen M
Journal: Journal of visualized experiments : JoVE (2016)
In Vivo Mapping of Eukaryotic RNA Interactomes Reveals Principles of Higher-Order Organization and Regulation.
Authors: Aw, Jong Ghut Ashley and Shen, Yang and Wilm, Andreas and Sun, Miao and Lim, Xin Ni and Boon, Kum-Loong and Tapsin, Sidika and Chan, Yun-Shen and Tan, Cheng-Peow and Sim, Adelene Y L and Zhang, Tong and Susanto, Teodorus Theo and Fu, Zhiyan and Nagarajan, Niranjan and Wan, Yue
Journal: Molecular cell (2016): 603-17
Authors: Aw, Jong Ghut Ashley and Shen, Yang and Wilm, Andreas and Sun, Miao and Lim, Xin Ni and Boon, Kum-Loong and Tapsin, Sidika and Chan, Yun-Shen and Tan, Cheng-Peow and Sim, Adelene Y L and Zhang, Tong and Susanto, Teodorus Theo and Fu, Zhiyan and Nagarajan, Niranjan and Wan, Yue
Journal: Molecular cell (2016): 603-17
RNA Duplex Map in Living Cells Reveals Higher-Order Transcriptome Structure.
Authors: Lu, Zhipeng and Zhang, Qiangfeng Cliff and Lee, Byron and Flynn, Ryan A and Smith, Martin A and Robinson, James T and Davidovich, Chen and Gooding, Anne R and Goodrich, Karen J and Mattick, John S and Mesirov, Jill P and Cech, Thomas R and Chang, Howard Y
Journal: Cell (2016): 1267-1279
Authors: Lu, Zhipeng and Zhang, Qiangfeng Cliff and Lee, Byron and Flynn, Ryan A and Smith, Martin A and Robinson, James T and Davidovich, Chen and Gooding, Anne R and Goodrich, Karen J and Mattick, John S and Mesirov, Jill P and Cech, Thomas R and Chang, Howard Y
Journal: Cell (2016): 1267-1279
Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability.
Authors: Colosio, Arianna and Frattini, Camilla and Pellicanò, Grazia and Villa-Hernández, Sara and Bermejo, Rodrigo
Journal: Nucleic acids research (2016): 10676-10690
Authors: Colosio, Arianna and Frattini, Camilla and Pellicanò, Grazia and Villa-Hernández, Sara and Bermejo, Rodrigo
Journal: Nucleic acids research (2016): 10676-10690
Repair of UV induced DNA lesions in ribosomal gene chromatin and the role of "Odd" RNA polymerases (I and III).
Authors: Charton, Romain and Guintini, Laetitia and Peyresaubes, François and Conconi, Antonio
Journal: DNA repair (2015): 49-58
Authors: Charton, Romain and Guintini, Laetitia and Peyresaubes, François and Conconi, Antonio
Journal: DNA repair (2015): 49-58