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Pyronin Y *CAS 92-32-0*

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Physical properties
Molecular weight302.8
Spectral properties
Absorbance (nm)550
Excitation (nm)547
Emission (nm)566
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Absorbance (nm)
Excitation (nm)
Emission (nm)
Pyronin Y is widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Pyronin Y *CAS 92-32-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM330.251 µL1.651 mL3.303 mL16.513 mL33.025 mL
5 mM66.05 µL330.251 µL660.502 µL3.303 mL6.605 mL
10 mM33.025 µL165.125 µL330.251 µL1.651 mL3.303 mL

Molarity calculator

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Spectral properties

Absorbance (nm)550
Excitation (nm)547
Emission (nm)566



View all 1 citations: Citation Explorer
Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957


View all 34 references: Citation Explorer
A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27
Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299
Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193
A methyl green-pyronin technique for demonstrating cell death in the murine tumour S180
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677
Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132
Dye standards, Part II. 1: Pyronin Y (CI 45005)
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220
Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305
1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209