Pyronin Y *CAS 92-32-0*
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Additional ordering information
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Physical properties
Molecular weight | 302.8 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 550 |
Excitation (nm) | 547 |
Emission (nm) | 566 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Nucleus
CAS 92-32-0 | Molecular weight 302.8 | Absorbance (nm) 550 | Excitation (nm) 547 | Emission (nm) 566 |
Pyronin Y is widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Pyronin Y *CAS 92-32-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 330.251 µL | 1.651 mL | 3.303 mL | 16.513 mL | 33.025 mL |
5 mM | 66.05 µL | 330.251 µL | 660.502 µL | 3.303 mL | 6.605 mL |
10 mM | 33.025 µL | 165.125 µL | 330.251 µL | 1.651 mL | 3.303 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 550 |
Excitation (nm) | 547 |
Emission (nm) | 566 |
Citations
View all 1 citations: Citation Explorer
Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957
References
View all 34 references: Citation Explorer
A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)
Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27
Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299
Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193
A methyl green-pyronin technique for demonstrating cell death in the murine tumour S180
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677
Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132
Dye standards, Part II. 1: Pyronin Y (CI 45005)
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220
Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305
1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?