Pyronin Y CAS 92-32-0

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Physical properties

Molecular weight	302.8
Solvent	DMSO

Spectral properties

Absorbance (nm)	550
Excitation (nm)	547
Emission (nm)	566

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

92-32-0

Molecular weight

302.8

Absorbance (nm)

550

Excitation (nm)

547

Emission (nm)

566

Pyronin Y is widely used in combination with methyl green to selectively and differentially stain nucleic acids following polyacrylamide gel electrophoresis. In a dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains DNA green.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Pyronin Y *CAS 92-32-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	330.251 µL	1.651 mL	3.303 mL	16.513 mL	33.025 mL
5 mM	66.05 µL	330.251 µL	660.502 µL	3.303 mL	6.605 mL
10 mM	33.025 µL	165.125 µL	330.251 µL	1.651 mL	3.303 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	550
Excitation (nm)	547
Emission (nm)	566

Images

Figure 1. Chemical structure for Pyronin Y *CAS 92-32-0*

Citations

View all 1 citations: Citation Explorer

Microfluidic Platform for the Isolation of Cancer-Cell Subpopulations Based on Single-Cell Glycolysis
Authors: Zielke, Claudia and Pan, Ching W and Gutierrez Ramirez, Adriana J and Feit, Cameron and Dobson, Chandler and Davidson, Catherine and Sandel, Brody and Abbyad, Paul
Journal: Analytical Chemistry (2020): 6949--6957

References

View all 34 references: Citation Explorer

A simple and high sensitive spectrophotometric method for ultra trace determination of ruthenium with its catalytic effect on the oxidation of pyronin B by periodate
Authors: Rezaei B, Majidi N.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)

Methyl green-pyronin Y staining of nucleic acids: studies on the effects of staining time, dye composition and diffusion rates
Authors: Prento P, Lyon HO.
Journal: Biotech Histochem (2003): 27

Pyronin Y as a fluorescent stain for paraffin sections
Authors: Li B, Wu Y, Gao XM.
Journal: Histochem J (2002): 299

Functional heterogeneity within rhodamine123(lo) Hoechst33342(lo/sp) primitive hemopoietic stem cells revealed by pyronin Y
Authors: Huttmann A, Liu SL, Boyd AW, Li CL.
Journal: Exp Hematol (2001): 1109

Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y
Authors: Toba K, Winton EF, Koike T, Shibata A.
Journal: J Immunol Methods (1995): 193

A methyl green-pyronin technique for demonstrating cell death in the murine tumour S180
Authors: Moffitt P., undefined
Journal: Cell Biol Int (1994): 677

Effects of mounting media on fading of toluidine blue and pyronin G staining in epoxy sections
Authors: Schmolke C., undefined
Journal: Biotech Histochem (1993): 132

Dye standards, Part II. 1: Pyronin Y (CI 45005)
Authors: European Committee for Clinical Laboratory St, undefined and ards (ECCLS), Subcommittee on Reference Materials for Tissue Stains (SRMTS)
Journal: Histochem J (1992): 220

Simultaneous quantification of DNA and RNA in tissue sections. A comparative analysis of the methyl green-pyronin technique with the gallocyanin chromalum and Feulgen procedures using image cytometry
Authors: Schulte EK, Lyon HO, Hoyer PE.
Journal: Histochem J (1992): 305

1-naphthol-pyronin B as a novel substrate for silver intensification: application to light and electron microscopic immunocytochemistry of neuroendocrine systems
Authors: Toni R, Lechan RM.
Journal: J Histochem Cytochem (1990): 1209