Name: Quin-2, AM *CAS 83104-85-2*
Brand: AAT Bioquest
SKU: 21050
Price: 207 USD
Availability: InStock

Quin-2, AM

CAS 83104-85-2

Quin-2 binds calcium tightly and resembles calcium chelator EGTA in ability to bind calcium much more tightly than magnesium. Binding of calcium causes large changes in ultraviolet absorption and fluorescence. The wavelengths of light that cause fluorescence when calcium is bound are longer than the wavelengths that cause fluorescence when it is not bound. When excited at two different wavelengths, the ratio of the fluorescence intensities at the two wavelengths gives the ratio of the concentrations of bound to free calcium. Free Quin-2 concentration can be measured precisely, so free calcium concentration can be calculated precisely. Quin-2 may be injected into cells to measure moment-to-moment changes in intracellular calcium concentration. Quin-2 AM is permeable to cells, and used for studying live cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21050	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Quin-2, AM *CAS 83104-85-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	120.517 µL	602.584 µL	1.205 mL	6.026 mL	12.052 mL
5 mM	24.103 µL	120.517 µL	241.034 µL	1.205 mL	2.41 mL
10 mM	12.052 µL	60.258 µL	120.517 µL	602.584 µL	1.205 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	829.76 g/mol	=	mL	x	mM	=	mmol

References

View all 60 references: Citation Explorer

W-5 and quin 2-AM reverse the inhibitory effect of insulin on lipolysis due to dibutyryl cAMP

Authors:

Goko H, Matsuoka A.

Journal:

Diabetes Res Clin Pract (1999): 101

Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells

Authors:

Faux SP, Michelangeli F, Levy LS.

Journal:

Mutat Res (1994): 209

Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2

Authors:

Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.

Journal:

Cell Calcium (1994): 7

Possible mechanisms of epinephrine actions in quin-2-loaded platelets refractory to arachidonic acid

Authors:

Rao GH, Gerrard JM, Murthy M, White JG.

Journal:

Biochem Med Metab Biol (1993): 322

Fluorescence lifetime imaging of calcium using Quin-2

Authors:

Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.

Journal:

Cell Calcium (1992): 131

Physical properties

Dissociation constant (K_d, nM)	60
Molecular weight	829.76
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	83104-85-2

Instrument settings

Fluorescence microplate reader
Excitation	340
Emission	495
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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