Fura-2 AM | AAT Bioquest

Fura-2, AM

UltraPure Grade; CAS 108964-32-5

Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2, AM is a cell-permeable calcium indicator that is emission-ratiometric and UV light'excitable. This AM ester form can be loaded into live cells noninvasively.

Fluorescence excitation spectra of Fura-2 in solutions conctaining 0 to 39uM free Ca2+.

Protocol

Catalog	Size	Price	Quantity
21021	1 mg	Price
21023	20x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fura-2, AM *UltraPure Grade* *CAS 108964-32-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	99.814 µL	499.072 µL	998.143 µL	4.991 mL	9.981 mL
5 mM	19.963 µL	99.814 µL	199.629 µL	998.143 µL	1.996 mL
10 mM	9.981 µL	49.907 µL	99.814 µL	499.072 µL	998.143 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1001.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 18 citations: Citation Explorer

A natural small molecule alleviates liver fibrosis by targeting apolipoprotein L2

Authors:

Gan, Lu and Jiang, Qiwei and Huang, Dong and Wu, Xueji and Zhu, Xinying and Wang, Lei and Xie, Wei and Huang, Jialuo and Fan, Runzhu and Jing, Yihang and others,

Journal:

Nature Chemical Biology (2024): 1--11

Calcium signals in the murine zona glomerulosa

Authors:

Dinh, Hoang An

Regulation of Calcium entry by cyclic GMP signaling in Toxoplasma gondii

Authors:

Triana, Miryam A Hortua and M{\'a}rquez-Nogueras, Karla M and Fazli, Mojtaba Sedigh and Quinn, Shannon and Moreno, Silvia NJ

Journal:

Journal of Biological Chemistry (2024): 105771

BK channels sustain neuronal Ca2+ oscillations to support hippocampal long-term potentiation and memory formation

Authors:

Pham, Thomas and Hussein, Tamara and Calis, Dila and Bischof, Helmut and Skrabak, David and Cruz Santos, Melanie and Maier, Selina and Sp{\"a}hn, David and Kalina, Daniel and Simonsig, Stefanie and others,

Journal:

Cellular and Molecular Life Sciences (2023): 369

Purinergic Signalling in M{\"u}ller Cells in the Context of Glaucomatous Neuroinflammation and Neurodegeneration

Authors:

Habib, Sofia

References

View all 119 references: Citation Explorer

Load of calcium probe Fura -2/AM in Escherichia coli cells

Authors:

Shao M, Wang HM, Liu ZH, Shen P, Cai RX.

Journal:

Wei Sheng Wu Xue Bao (2005): 805

An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell

Authors:

McHugh JM, Kenyon JL.

Journal:

Am J Physiol Cell Physiol (2004): C342

Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2

Authors:

Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.

Journal:

J Fluoresc (2004): 711

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Authors:

McConnell G, Riis E.

Journal:

Phys Med Biol (2004): 4757

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2

Authors:

Xu T, Yang W, Huo XL, Song T.

Journal:

J Biochem Biophys Methods (2004): 219

Physical properties

Dissociation constant (K_d, nM)	145
Molecular weight	1001.86
Solvent	DMSO

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	108964-32-5

Instrument settings

Fluorescence microscope
Excitation	Fura 2 filter set
Emission	Fura 2 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	340, 380
Emission	510
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet (Catalog 21021)
Safety Data Sheet (Catalog 21023)
Certificate of Origin
Certificate of Analysis
Brochure: Calcium Detection Probes & Assay Kits
Brochure: Physiological Probes & Assay Kits

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Page updated on October 10, 2024