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Fura-2, AM
UltraPure Grade; CAS 108964-32-5
Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. Fura-2, AM is a cell-permeable calcium indicator that is emission-ratiometric and UV light'excitable. This AM ester form can be loaded into live cells noninvasively.
Fluorescence excitation spectra of Fura-2 in solutions conctaining 0 to 39uM free Ca2+.
Fluorescence excitation spectra of Fura-2 in solutions conctaining 0 to 39uM free Ca2+.
CatalogSize
Price
Quantity
210211 mg
Price
2102320x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)145
Molecular weight1001.86
SolventDMSO
Spectral properties

Excitation (nm)336
Emission (nm)505
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS108964-32-5
Instrument settings

Fluorescence microscope
ExcitationFura 2 filter set
EmissionFura 2 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation340, 380
Emission510
Cutoff475
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 10, 2024