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Fura-8™, AM
Although Fura-2 has become the preferred excitation-ratioable calcium indicator of choice, it has certain limitations (e.g., lower sensitivity than single wavelength calcium indicators such as Fluo-8® and Cal-520®). To address these concerns, AAT Bioquest has devoted considerable efforts to the development of Fura™ 8, a high-affinity ratiometric calcium indicator with improved sensitivity and higher signal-to-noise ratios. The fluorescence emission of Fura™ 8 is red-shifted to a longer visible wavelength, facilitating the detection of Fura™ 8 by common filter sets. Fura™ 8, AM is membrane-permeant and is excited at 355 nm and 415 nm and emits at 530 nm.
ATP Dose response in CHO-K1 cells measured with Fura-2 AM and Fura-8™ AM respectively. CHO-K1 cells were seeded overnight at 40,000 cells/100 µL/well in a black wall/clear bottom 96-well plate. The cells incubated with Fura-2 AM or Fura-8 AM calcium assay  dye-loading solution respectively for 1 hour. ATP Dose was added by Flexstation.
ATP Dose response in CHO-K1 cells measured with Fura-2 AM and Fura-8™ AM respectively. CHO-K1 cells were seeded overnight at 40,000 cells/100 µL/well in a black wall/clear bottom 96-well plate. The cells incubated with Fura-2 AM or Fura-8 AM calcium assay  dye-loading solution respectively for 1 hour. ATP Dose was added by Flexstation.
CatalogSize
Price
Quantity
210551 mg
Price
2105610x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)260
Molecular weight951.90
SolventDMSO
Spectral properties

Excitation (nm)354
Emission (nm)524
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationFura 2 filter set
EmissionFura 2 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation355, 415
Emission530
Cutoff475
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 13, 2025