Fura-8™, AM

Although Fura-2 has become the preferred excitation-ratioable calcium indicator of choice, it has certain limitations (e.g., lower sensitivity than single wavelength calcium indicators such as Fluo-8® and Cal-520®). To address these concerns, AAT Bioquest has devoted considerable efforts to the development of Fura™ 8, a high-affinity ratiometric calcium indicator with improved sensitivity and higher signal-to-noise ratios. The fluorescence emission of Fura™ 8 is red-shifted to a longer visible wavelength, facilitating the detection of Fura™ 8 by common filter sets. Fura™ 8, AM is membrane-permeant and is excited at 355 nm and 415 nm and emits at 530 nm.

Protocol

COA

Catalog	Size	Price	Quantity
21055	1 mg	Price
21056	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fura-8™, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	105.053 µL	525.265 µL	1.051 mL	5.253 mL	10.505 mL
5 mM	21.011 µL	105.053 µL	210.106 µL	1.051 mL	2.101 mL
10 mM	10.505 µL	52.527 µL	105.053 µL	525.265 µL	1.051 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	951.9 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Antagonistic control of intracellular signals by EpOMEs in hemocytes induced by PGE2 and their chemical modification for a potent insecticide

Authors:

Shahmohammadi, Niayesh and Haraji, Shiva and Khan, Falguni and S{\o}rsk{\aa}r, {\AA}shild Moi and Danielsen, Parastoo Ebrahimi and Vik, Anders and Kim, Yonggyun

Journal:

PloS one (2025): e0320488

TRPM8 overexpression suppresses hepatocellular carcinoma progression and improves survival by modulating the RTP3/STAT3 pathway

Authors:

Chen, Lichan and Xu, Nansong and Gou, DongMei and Song, Jianning and Zhou, Mingqin and Zhang, Yajun and Zhang, Haohua and Zhu, Liwen and Huang, Weihong and Zhu, Yue and others,

Journal:

Cancer Medicine (2024): e70109

HMGB1-Like Dorsal Switch Protein 1 Triggers a Damage Signal in Mosquito Gut to Activate Dual Oxidase via Eicosanoids

Authors:

Ahmed, Shabbir and Sajjadian, Seyedeh Minoo and Kim, Yonggyun

Journal:

Journal of Innate Immunity (2022): 1--16

Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin

Authors:

Harada, Naoki and Okuyama, Mai and Teraoka, Yoshiaki and Arahori, Yumi and Shinmori, Yoh and Horiuchi, Hiroko and Luis, Paula B and Joseph, Akil I and Kitakaze, Tomoya and Matsumura, Shigenobu and others,

Journal:

npj Science of Food (2022): 1--9

PGE2 mediates hemocyte-spreading behavior by activating aquaporin via cAMP and rearranging actin cytoskeleton via Ca2+

Authors:

Ahmed, Shabbir and Kim, Yonggyun

Journal:

Developmental \& Comparative Immunology (2021): 104230

References

View all 84 references: Citation Explorer

Measurement of [Ca2+] in cell suspensions using indo-1

Authors:

Nelemans A., undefined

Journal:

Methods Mol Biol (2006): 47

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Ratiometric intracellular calcium imaging in the isolated beating rat heart using indo-1 fluorescence

Authors:

Eerbeek O, Mik EG, Zuurbier CJ, van 't Loo M, Donkersloot C, Ince C.

Journal:

J Appl Physiol (2004): 2042

Negative inotropic effects of angiotensin II, endothelin-1 and phenylephrine in indo-1 loaded adult mouse ventricular myocytes

Authors:

Sakurai K, Norota I, Tanaka H, Kubota I, Tomoike H, Endo M.

Journal:

Life Sci (2002): 1173

Usefulness of the analytic method of intracellular calcium and the problems--aequorin and indo-1 signal

Authors:

Endoh M., undefined

Journal:

Nippon Yakurigaku Zasshi (2000): 361

Physical properties

Dissociation constant (K_d, nM)	260
Molecular weight	951.90
Solvent	DMSO

Spectral properties

Excitation (nm)	354
Emission (nm)	524

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microscope
Excitation	Fura 2 filter set
Emission	Fura 2 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	355, 415
Emission	530
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet (Catalog 21055)
Safety Data Sheet (Catalog 21056)
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 22, 2025