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Fura-FF, AM
Fura-2FF, AM; CAS 348079-12-9
Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. The cell-permeant Fura-2FF AM is an analog of Fura-2 AM with much lower calcium binding affinity, Kd ~10 µM. This AM ester form can be loaded into live cells noninvasively.
Fluorescence excitation spectra of Fura-2™ in the presence of 0 to 39 µM free Ca2+.
Fluorescence excitation spectra of Fura-2™ in the presence of 0 to 39 µM free Ca2+.
CatalogSize
Price
Quantity
2102710x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)5500
Molecular weight1023.80
SolventDMSO
Spectral properties

Excitation (nm)336
Emission (nm)505
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS348079-12-9
Instrument settings

Fluorescence microscope
ExcitationFura 2 filter set
EmissionFura 2 filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation340, 380
Emission510
Cutoff475
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 10, 2025