Name: Fura-FF, AM [Fura-2FF, AM] *CAS 348079-12-9*
Brand: AAT Bioquest
SKU: 21027
Price: 232 USD
Availability: InStock

Fura-FF, AM

Fura-2FF, AM; CAS 348079-12-9

Among the ratiometric calcium indicators, Fura-2 and Indo-1 are most commonly used. Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable. Fura-2 is preferred for ratio-imaging microscopy, in which it is more practical to change excitation wavelengths than emission wavelengths. Upon binding Ca2+, Fura-2 exhibits an absorption shift that can be observed by scanning the excitation spectrum between 300 and 400 nm, while monitoring the emission at ~510 nm. The cell-permeant Fura-2FF AM is an analog of Fura-2 AM with much lower calcium binding affinity, Kd ~10 µM. This AM ester form can be loaded into live cells noninvasively.

Fluorescence excitation spectra of Fura-2™ in the presence of 0 to 39 µM free Ca2+.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21027	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fura-FF, AM [Fura-2FF, AM] *CAS 348079-12-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	97.675 µL	488.377 µL	976.753 µL	4.884 mL	9.768 mL
5 mM	19.535 µL	97.675 µL	195.351 µL	976.753 µL	1.954 mL
10 mM	9.768 µL	48.838 µL	97.675 µL	488.377 µL	976.753 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1023.8 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

An Orai1 gain-of-function tubular aggregate myopathy mouse model phenocopies key features of the human disease

Authors:

Zhao, Nan and Michelucci, Antonio and Pietrangelo, Laura and Malik, Sundeep and Groom, Linda and Leigh, Jennifer and O’Connor, Thomas N and Takano, Takahiro and Kingsley, Paul D and Palis, James and others,

Journal:

The EMBO Journal (2024): 1--31

Endurance exercise attenuates juvenile irradiation-induced skeletal muscle functional decline and mitochondrial stress

Authors:

O’Connor, Thomas N and Kallenbach, Jacob G and Orciuoli, Haley M and Paris, Nicole D and Bachman, John F and Johnston, Carl J and Hernady, Eric and Williams, Jacqueline P and Dirksen, Robert T and Chakkalakal, Joe V

Journal:

Skeletal Muscle (2022): 1--15

Orai1--STIM1 Regulates Increased Ca2+ Mobilization, Leading to Contractile Duchenne Muscular Dystrophy Phenotypes in Patient-Derived Induced Pluripotent Stem Cells

Authors:

Uchimura, Tomoya and Sakurai, Hidetoshi

Journal:

Biomedicines (2021): 1589

An essential role of NAD (P) H oxidase 2 in UVA-induced calcium oscillations in mast cells

Authors:

Li, Zhi Ying and Jiang, Wen Yi and Cui, Zong Jie

Journal:

Photochemical & Photobiological Sciences (2015): 414--428

Lasting inhibition of receptor-mediated calcium oscillations in pancreatic acini by neutrophil respiratory burst--A novel mechanism for secretory blockade in acute pancreatitis?

Authors:

Liang, Hui Yuan and Song, Zhi Min and Cui, Zong Jie

Journal:

Biochemical and biophysical research communications (2013): 361--367

References

View all 119 references: Citation Explorer

Load of calcium probe Fura -2/AM in Escherichia coli cells

Authors:

Shao M, Wang HM, Liu ZH, Shen P, Cai RX.

Journal:

Wei Sheng Wu Xue Bao (2005): 805

An Excel-based model of Ca2+ diffusion and fura 2 measurements in a spherical cell

Authors:

McHugh JM, Kenyon JL.

Journal:

Am J Physiol Cell Physiol (2004): C342

Problems caused by high concentration of ATP on activation of the P2X7 receptor in bone marrow cells loaded with the Ca2+ fluorophore fura-2

Authors:

Paredes-Gamero EJ, Franca JP, Moraes AA, Aguilar MO, Oshiro ME, Ferreira AT.

Journal:

J Fluoresc (2004): 711

Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with fura-2

Authors:

McConnell G, Riis E.

Journal:

Phys Med Biol (2004): 4757

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2

Authors:

Xu T, Yang W, Huo XL, Song T.

Journal:

J Biochem Biophys Methods (2004): 219

Physical properties

Dissociation constant (K_d, nM)	5500
Molecular weight	1023.80
Solvent	DMSO

Spectral properties

Excitation (nm)	336
Emission (nm)	505

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	348079-12-9

Instrument settings

Fluorescence microscope
Excitation	Fura 2 filter set
Emission	Fura 2 filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	340, 380
Emission	510
Cutoff	475
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 20, 2025