Name: Fura Red, AM *CAS 149732-62-7*
Brand: AAT Bioquest
SKU: 21046
Availability: InStock

Fura Red, AM

CAS 149732-62-7

Fura Red is a visible light-excitable fura-2 analog that offers unique possibilities for ratiometric measurement of calcium ion in single cells by microphotometry, imaging or flow cytometry when used with single excitation, green-fluorescent calcium indicators. Fura Red AM is the cell-permeable version of Fura Red used for noninvasive intracellular loading. Fura Red AM can be simultaneously loaded into cells with Fluo-3 AM, Fluo-8 AM or Cal-520 AM. An advantage of combining two calcium dyes is that dyes with longer excitation wavelengths can be used. This usually causes less harm to the cells than using ratiometric dyes that are excited with UV- or near UV-light (e.g. Fura-2), as light at visible wavelengths is less phototoxic.

Protocol

COA

Catalog	Size	Price	Quantity
21046	1 mg	Price
21048	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fura Red, AM *CAS 149732-62-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	91.828 µL	459.141 µL	918.282 µL	4.591 mL	9.183 mL
5 mM	18.366 µL	91.828 µL	183.656 µL	918.282 µL	1.837 mL
10 mM	9.183 µL	45.914 µL	91.828 µL	459.141 µL	918.282 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1088.99 g/mol	=	mL	x	mM	=	mmol

Citations

View all 9 citations: Citation Explorer

Renal Implications of Kappa Opioid Receptor Signaling in Sprague-Dawley Rats

Authors:

Didik, Steven and Golosova, Daria and Zietara, Adrian and Bohovyk, Ruslan and Ahrari, Ameneh and Levchenko, Vladislav and Kravtsova, Olha and Taneja, Krish and Khedr, Sherif and Semenikhina, Marharyta and others,

Journal:

Function (2025): zqaf028

Ultrasound-mediated spatial and temporal control of engineered cells in vivo

Authors:

Ivanovski, Filip and Me{\v{s}}ko, Maja and Lebar, Tina and Rupnik, Marko and Lain{\v{s}}{\v{c}}ek, Du{\v{s}}ko and Gradi{\v{s}}ek, Miha and Jerala, Roman and Ben{\v{c}}ina, Mojca

Journal:

Nature Communications (2024): 7369

P-selectin-dependent leukocyte adhesion is governed by endolysosomal two-pore channel 2

Authors:

Goretzko, Jonas and Pauels, Inga and Heitzig, Nicole and Thomas, Katharina and Kardell, Marina and Na{\ss}, Johannes and Krogsaeter, Einar Kleinhans and Schloer, Sebastian and Spix, Barbara and Matos, Anna L{\'\i}via Linard and others,

Journal:

Cell Reports (2023): 113501

Acute and long-term effects of cannabinoids on hypertension and kidney injury

Authors:

Golosova, Daria and Levchenko, Vladislav and Kravtsova, Olha and Palygin, Oleg and Staruschenko, Alexander

Journal:

Scientific reports (2022): 1--12

Arachidonic acid-regulated calcium signaling in T cells from patients with rheumatoid arthritis promotes synovial inflammation

Authors:

Ye, Zhongde and Shen, Yi and Jin, Ke and Qiu, Jingtao and Hu, Bin and Jadhav, Rohit R and Sheth, Khushboo and Weyand, Cornelia M and Goronzy, J{\"o}rg J

Journal:

Nature communications (2021): 1--17

References

View all 12 references: Citation Explorer

Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets

Authors:

Wendt ER, Ferry H, Greaves DR, Keshav S.

Journal:

PLoS One (2015): e0119532

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue

Authors:

Walczysko P, Wagner E, Albrechtova JT.

Journal:

Cell Calcium (2000): 23

Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red

Authors:

Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.

Journal:

Shi Yan Sheng Wu Xue Bao (1998): 323

Problems associated with using Fura-2 to measure free intracellular calcium concentrations in human red blood cells

Authors:

Blackwood AM, Sagnella GA, Mark and u ND, MacGregor GA.

Journal:

J Hum Hypertens (1997): 601

Physical properties

Dissociation constant (K_d, nM)	400
Molecular weight	1088.99
Solvent	DMSO

Spectral properties

Excitation (nm)	435
Emission (nm)	639

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	149732-62-7

Instrument settings

Fluorescence microplate reader
Excitation	435, 470
Emission	630, 650
Cutoff	Ex/Em = 435/630, cutoff 610. Ex/Em = 470/650, cut off 630
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 6, 2025