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Rhod-FF, AM
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-FF AM is cell-permeable, and generates Rhod-FF upon esterase hydrolysis in cells. Rhod-FF has a lower binding affinity for Ca2+ and is suitable for Ca2+ measurements from 10 to 200 uM. Like the parent Rhod-2 indicator, Rhod-FF is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+.
Chemical structure for Rhod-FF, AM
Chemical structure for Rhod-FF, AM
CatalogSize
Price
Quantity
210771 mg
Price
2107810x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)19000
Molecular weight1145.90
SolventDMSO
Spectral properties

Excitation (nm)553
Emission (nm)577
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationTRITC filter set
EmissionTRITC filter set
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation540
Emission590
Cutoff570
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 6, 2025