Name: Rhod-FF, AM
Brand: AAT Bioquest
SKU: 21077
Availability: InStock

Rhod-FF, AM

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Rhod-FF AM is cell-permeable, and generates Rhod-FF upon esterase hydrolysis in cells. Rhod-FF has a lower binding affinity for Ca2+ and is suitable for Ca2+ measurements from 10 to 200 uM. Like the parent Rhod-2 indicator, Rhod-FF is essentially nonfluorescent in the absence of divalent cations and exhibits strong fluorescence enhancement with no spectral shift upon binding Ca2+.

Protocol

Catalog	Size	Price	Quantity
21077	1 mg	Price
21078	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhod-FF, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.268 µL	436.338 µL	872.676 µL	4.363 mL	8.727 mL
5 mM	17.454 µL	87.268 µL	174.535 µL	872.676 µL	1.745 mL
10 mM	8.727 µL	43.634 µL	87.268 µL	436.338 µL	872.676 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1145.9 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Activation of mitochondrial transient receptor potential vanilloid 1 channel contributes to microglial migration

Authors:

Miyake, Takahito and Shirakawa, Hisashi and Nakagawa, Takayuki and Kaneko, Shuji

Journal:

Glia (2015): 1870--1882

ER stress in human hepatic cells treated with Efavirenz: mitochondria again

Authors:

Apostolova, Nadezda and Gomez-Sucerquia, Leysa J and Alegre, Fern and o , undefined and Funes, Haryes A and Victor, Victor M and Barrachina, Maria D and Blas-Garcia, Ana and Esplugues, Juan V

Journal:

Journal of hepatology (2013): 780--789

Physical properties

Dissociation constant (K_d, nM)	19000
Molecular weight	1145.90
Solvent	DMSO

Spectral properties

Excitation (nm)	553
Emission (nm)	577

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	TRITC filter set
Emission	TRITC filter set
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	540
Emission	590
Cutoff	570
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on July 18, 2026