Quin-2, tetrapotassium salt *CAS 149022-19-5*
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Physical properties
Dissociation constant (Kd, nM) | 60 |
Molecular weight | 693.87 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Calcium Indicators
CAS 149022-19-5 | Molecular weight 693.87 | Dissociation constant (Kd, nM) 60 |
Quin-2 binds calcium tightly and resembles calcium chelator EGTA in ability to bind calcium much more tightly than magnesium. Binding of calcium causes large changes in ultraviolet absorption and fluorescence. The wavelengths of light that cause fluorescence when calcium is bound are longer than the wavelengths that cause fluorescence when it is not bound. When excited at two different wavelengths, the ratio of the fluorescence intensities at the two wavelengths gives the ratio of the concentrations of bound to free calcium. Free Quin-2 concentration can be measured precisely, so free calcium concentration can be calculated precisely. Quin-2 may be injected into cells to measure moment-to-moment changes in intracellular calcium concentration. Quin-2 AM is permeable to cells, and used for studying live cells.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Quin-2, tetrapotassium salt *CAS 149022-19-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 144.119 µL | 720.596 µL | 1.441 mL | 7.206 mL | 14.412 mL |
5 mM | 28.824 µL | 144.119 µL | 288.238 µL | 1.441 mL | 2.882 mL |
10 mM | 14.412 µL | 72.06 µL | 144.119 µL | 720.596 µL | 1.441 mL |
Molarity calculator
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References
View all 60 references: Citation Explorer
W-5 and quin 2-AM reverse the inhibitory effect of insulin on lipolysis due to dibutyryl cAMP
Authors: Goko H, Matsuoka A.
Journal: Diabetes Res Clin Pract (1999): 101
Authors: Goko H, Matsuoka A.
Journal: Diabetes Res Clin Pract (1999): 101
Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells
Authors: Faux SP, Michelangeli F, Levy LS.
Journal: Mutat Res (1994): 209
Authors: Faux SP, Michelangeli F, Levy LS.
Journal: Mutat Res (1994): 209
Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.
Journal: Cell Calcium (1994): 7
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.
Journal: Cell Calcium (1994): 7
Possible mechanisms of epinephrine actions in quin-2-loaded platelets refractory to arachidonic acid
Authors: Rao GH, Gerrard JM, Murthy M, White JG.
Journal: Biochem Med Metab Biol (1993): 322
Authors: Rao GH, Gerrard JM, Murthy M, White JG.
Journal: Biochem Med Metab Biol (1993): 322
Fluorescence lifetime imaging of calcium using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.
Journal: Cell Calcium (1992): 131
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.
Journal: Cell Calcium (1992): 131
Toxicity to isolated hepatocytes caused by the intracellular calcium indicator, Quin 2
Authors: Carpenter-Deyo L, Duimstra JR, Hedstrom O, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 739
Authors: Carpenter-Deyo L, Duimstra JR, Hedstrom O, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 739
Involvement of calcium and iron in Quin 2 toxicity to isolated hepatocytes
Authors: Carpenter-Deyo L, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 747
Authors: Carpenter-Deyo L, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 747
Aspirin, prostaglandin E1 and Quin-2 AM-induced platelet dysfunction: restoration of function by noradrenalin
Authors: Rao GH, White JG.
Journal: Prostaglandins Leukot Essent Fatty Acids (1990): 141
Authors: Rao GH, White JG.
Journal: Prostaglandins Leukot Essent Fatty Acids (1990): 141
Effect of Quin-2 on Ca2+ uptake mediated by Na+i/Ca2+o exchange and 45Ca2+ efflux in rat brain synaptosomes: a requirement for [Ca2+]i
Authors: Blanco P, Martinez-Serrano A, Bogonez E, Satrustegui J.
Journal: Cell Calcium (1990): 25
Authors: Blanco P, Martinez-Serrano A, Bogonez E, Satrustegui J.
Journal: Cell Calcium (1990): 25
Quin-2 and fura-2 measure calcium differently
Authors: Mazorow DL, Millar DB.
Journal: Anal Biochem (1990): 28
Authors: Mazorow DL, Millar DB.
Journal: Anal Biochem (1990): 28
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
Novel Red Fluorescent Calcium Probes for Functional Analysis of GPCRs and Calcium Channel Targets
FAQ
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?
How do you measure Ca2+ response with calcium indicators like Calbryte 520, potassium salt?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?