Quin-2, tetrapotassium salt *CAS 149022-19-5*

Quin-2, tetrapotassium salt

CAS 149022-19-5

Quin-2 binds calcium tightly and resembles calcium chelator EGTA in ability to bind calcium much more tightly than magnesium. Binding of calcium causes large changes in ultraviolet absorption and fluorescence. The wavelengths of light that cause fluorescence when calcium is bound are longer than the wavelengths that cause fluorescence when it is not bound. When excited at two different wavelengths, the ratio of the fluorescence intensities at the two wavelengths gives the ratio of the concentrations of bound to free calcium. Free Quin-2 concentration can be measured precisely, so free calcium concentration can be calculated precisely. Quin-2 may be injected into cells to measure moment-to-moment changes in intracellular calcium concentration. Quin-2 AM is permeable to cells, and used for studying live cells.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21052	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Quin-2, tetrapotassium salt *CAS 149022-19-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	144.119 µL	720.596 µL	1.441 mL	7.206 mL	14.412 mL
5 mM	28.824 µL	144.119 µL	288.238 µL	1.441 mL	2.882 mL
10 mM	14.412 µL	72.06 µL	144.119 µL	720.596 µL	1.441 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	693.87 g/mol	=	mL	x	mM	=	mmol

References

View all 60 references: Citation Explorer

W-5 and quin 2-AM reverse the inhibitory effect of insulin on lipolysis due to dibutyryl cAMP

Authors:

Goko H, Matsuoka A.

Journal:

Diabetes Res Clin Pract (1999): 101

Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells

Authors:

Faux SP, Michelangeli F, Levy LS.

Journal:

Mutat Res (1994): 209

Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2

Authors:

Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.

Journal:

Cell Calcium (1994): 7

Possible mechanisms of epinephrine actions in quin-2-loaded platelets refractory to arachidonic acid

Authors:

Rao GH, Gerrard JM, Murthy M, White JG.

Journal:

Biochem Med Metab Biol (1993): 322

Fluorescence lifetime imaging of calcium using Quin-2

Authors:

Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.

Journal:

Cell Calcium (1992): 131

Physical properties

Dissociation constant (K_d, nM)	60
Molecular weight	693.87
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	149022-19-5

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